Name: Anti-P2Y14 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: APR-018-F

Overview

Cat #: APR-018-F

Alternative Name P2Y purinoceptor 14, P2RY14, GPR105, UDP-glucose receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CIELKSELGRKWHKASN, corresponding to amino acid residues 172-188 of human P2RY14 (Accession Q15391). Extracellular, 2^nd loop.

Accession (Uniprot) Number Q15391

Gene ID 9934

Homology Rat, mouse – 15 out of 17 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-018), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of P2Y14 by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC
^___ Cells + Anti-P2Y14 (extracellular)-FITC Antibody (#APR-018-F), (2.5µg)
Cell surface detection of P2Y14 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC
^___ Cells + Anti-P2Y14 (extracellular)-FITC Antibody (#APR-018-F), (5µg)

References

Chambers, J.K. et al. (2000) J. Biol. Chem. 275, 10767.
Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
Skelton, L. et al. (2003) J. Immunol. 171, 1941.

Scientific background

P2Y14 belongs to the P2Y receptor family that is part of the G-protein coupled receptor superfamily. To date, eight functional mammalian P2Y receptors have been identified: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13 and P2Y14.

The P2Y receptor family mediates the effects of extracellular nucleotides such as ATP, ADP, UTP, and in the case of P2Y14, UDP-glucose. UDP-glucose, as with the other nucleotides, can be released to the extracellular milieu as the result of cell damage or by regulated non-lytic mechanisms.

P2Y14 is expressed in several tissues including the brain, lung and several cells of hematopoietic origin such as T lymphocytes and dendritic cells.

The exact function of this receptor in the different tissues has not been clearly established but an involvement in chemotaxis, maturation of immature dendritic cells and neuroimmune regulation has been suggested.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2Y14 (extracellular) Antibody (#APR-018) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize P2RY14 from human, mouse, and rat samples

Anti-P2Y14 (extracellular)-FITC Antibody (#APR-018-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-P2Y14 (extracellular)-FITC Antibody