Overview
- Peptide CIELKSELGRKWHKASN, corresponding to amino acid residues 172-188 of human P2RY14 (Accession Q15391). 2nd extracellular loop.
- Rat brain and lung lysates, and rat heart membrane (1:200).
Western blot analysis of rat heart membranes (lanes 1 and 4), brain (lanes 2 and 5) and lung lysates (lanes 3 and 6):1-3. Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018), (1:200).
4-6. Anti-P2Y14 Receptor (extracellular) Antibody, preincubated with P2Y14 Receptor (extracellular) Blocking Peptide (#BLP-PR018).
- Mouse kidney sections (Azroyan, A. et al. (2015) PLoS ONE 10, e0121419.).
- Human THP-1 monocytic leukemia cells (5 μg).
P2Y14 belongs to the P2Y receptor family that is part of the G-protein coupled receptor superfamily. To date, eight functional mammalian P2Y receptors have been identified: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13 and P2Y14.
The P2Y receptor family mediates the effects of extracellular nucleotides such as ATP, ADP, UTP, and in the case of P2Y14, UDP-glucose. UDP-glucose, as with the other nucleotides, can be released to the extracellular milieu as the result of cell damage or by regulated non-lytic mechanisms.
P2Y14 is expressed in several tissues including the brain, lung and several cells of hematopoietic origin such as T lymphocytes and dendritic cells.
The exact function of this receptor in the different tissues has not been clearly established but an involvement in chemotaxis, maturation of immature dendritic cells and neuroimmune regulation has been suggested.
Expression of P2Y14 receptor in mouse kidney.Immunohistochemical staining of mouse kidney sections using Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018). P2RY14 staining (green) is detected in intercalated cells in the cortex and co-localizes with V-ATPase B1 subunit (red).Adapted from Azroyan, A. et al. (2015) PLoS ONE 10, e0121419. with permission of PLoS.
