Overview
- Peptide (C)KPAY GTTGL PRAKR KSVR, corresponding to residues 227-244 of rat P2RY2 (Accession P41232). 3rd intracellular loop (i3) between TM5 and TM6 domains.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1, 2. Anti-P2Y2 Receptor Antibody (#APR-010), (1:200).
3, 4. Anti-P2Y2 Receptor Antibody, preincubated with P2Y2 Receptor Blocking Peptide (#BLP-PR010).- Mouse mIMCD-K2 cell lysate (4 μg/ml) (Rajagopal, M. et al. (2011) Am. J. Physiol. 301, F544.).
- Rat DRGs (Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.).
- Rat dorsal root ganglia (1:300) (Mo, G. et al. (2013) Mol. Pharmacol. 83, 640.).
- Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
- Rat PC12 cells (1:100) (Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.).
- Rat osteoclasts (8 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
- The control antigen is not suitable for this application.
- Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
- Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
- Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
- Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
- Di Virgilio, F. et al. (2001) Blood 97, 587.
- Yerxa, B.R. et al. (2002) J. Pharmacol. Exp. Ther. 302, 871.
The P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1
The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14 .2-4
The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway.5 It has become a leading candidate for the treatment of cystic fibrosis. Activation of P2Y2 receptor increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.6
Anti-P2Y2 Receptor Antibody (#APR-010) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY2 in rat, mouse, and human samples.
Expression of P2Y2 receptor in rat cortical neurons.Immunocytochemical staining of rat cortical neurons using Anti-P2Y2 Receptor Antibody (#APR-010). P2Y2 receptor immuno-colocalizes with PSD-95, the synaptic marker.Adapted from Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50. with permission of the American Society for Pharmacology & Experimental Therapeutics.
Applications
Citations
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Multiplex staining of KCa3.1 and P2Y2 in human ovarian carcinomaImmunohistochemical staining of human ovarian carcinoma biopsies using Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) and rabbit Anti-P2Y2 Receptor Antibody (#APR-010). Positive co-expression is detected in ovarian neoplastic cells (merged image). Expression of KCa3.1 is also observed in stromal cells (*); however, P2RY2 does not co-localize with KCa3.1 in these cells.
Adapted from Robles-Martinez, L. et al. (2017) Sci. Rep. 7, 4340. with permission of Nature Publishing Group.
- Western blot analysis of human umbilical vein endothelial cells (HUVECs). Tested in siRNA-treated cells.
Sathanoori, R. et al. (2017) Cell. Mol. Life Sci. 74, 731.
- Human umbilical vein endothelial cells (HUVECs). Tested in siRNA-treated cells.
Sathanoori, R. et al. (2017) Cell. Mol. Life Sci. 74, 731. - Rat osteoclast lysate.
Ellegaard, M. et al. (2017) Purinergic Signal. 13, 545. - Rat primary astrocyte culture lysate.
Paniagua-Herranz, L. et al. (2017) Front. Pharmacol. 8, 937. - Human esophageal epithelial cell lysate.
Wu, L. et al. (2017) J. Gastroenrol. Hepatol. 32, 1341. - Mouse brain lysate.
Cui, J.D. et al. (2016) Chem. Biol. Interact. 259, 291. - Mouse bone marrow lysate.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Mouse bone lysate and osteoblast MOB-C cell lysate.
Seref-Ferlengez, Z. et al. (2016) PLoS ONE 11, e0155107. - Rat cardiac fibloblast lysate (1:200).
Certal, M. et al. (2015) Cell Calcium 58, 518. - HEK 293 transfected cell lysates.
Yashima, S. et al. (2015) J. Biochem. 158, 155. - Human MCF-7 cell lysate (1:200).
Chadet, S. et al. (2014) Carcinogenesis 35, 1238. - Rat cortical neuron lysate (1:1000).
Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50. - Mouse cerebral synaptosome.
Naviaux, R.K. et al. (2013) PLoS ONE 8, e57380. - Mouse mIMCD-K2 cell lysate (4 μg/ml).
Rajagopal, M. et al. (2011) Am. J. Physiol. 301, F544.
- Mouse bone marrow lysate.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Rat DRGs.
Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.
- Rat kidney sections.
Palygin, O. et al. (2018) Punergic Signal. 14, 485. - Mouse anterior pole eye sections.
Fonseca, B. et al. (2017) Front. Pharmacol. 8, 719. - Human ovarian tumor sections.
Robles-Martinez, L. et al. (2017) Sci. Rep. 7, 4340. - Rat spinal cord sections (1:150).
Shi, Y. et al. (2017) Gene 627, 263. - Human esophagus sections.
Wu, L. et al. (2017) J. Gastroenrol. Hepatol. 32, 1341. - Mouse hippocampal slices (1:50).
Takano, T. et al. (2014) Glia 62, 78. - Rat dorsal root ganglia (1:300).
Mo, G. et al. (2013) Mol. Pharmacol. 83, 640. - Human eye tissue.
Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.
- Rat cardiac fibroblasts (1:100).
Certal, M. et al. (2015) Cell Calcium 58, 518. - Rat cortical neurons (1:200).
Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50. - HEK-293T cells transfected with rat P2Y2R (1:200).
Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50. - Rat PC12 cells (1:100).
Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.
- Mouse bone marrow cells.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Rat osteoclasts (8 μg/ml).
Alvarenga, E.C. et al. (2010) Bone 46, 355.
- Traves, P.G. et al. (2013) J. Immunol. 190, 4226.
- Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
- Mortensen, S.P. et al. (2012) J. Physiol. 590, 6227.
