Overview
Alternative Name P2Y purinoceptor 6, P2RY6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)RNRTV(S)YDLSPPILS, corresponding to amino acid residues 172 - 186 of mouse P2RY6 (Accession Q9ERK9). 2nd extracellular loop.
Accession (Uniprot) Number Q9ERK9
Gene ID 233571
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) heart membranes:1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:400).
3,4. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).
Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).
3,4. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).
Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate:1. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).
2. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).
Immunohistochemistry
Expression of P2RY6 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:1000), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. P2RY6 immunoreactivity (red) appears in pyramidal neurons (arrows). Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
Cell surface detection of P2RY6 live intact mouse J774 macrophage cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (2.5 µg) + goat-anti-rabbit-FITC.
References
- Communi, D. et al. (2000) Cell Signal. 12, 351.
- Filippov, A.K. et al. (1999) Br. J. Pharmacol. 126, 1009.
- Chang, K. et al. (1995) J. Biol. Chem. 270, 26152.
- Malmsjo, M. et al. (2003) B.M.C. Pharmacol. 3, 4.
Scientific background
The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily1,3.
P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels1,2.
P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries4.
Lyophilized Powder
Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry applications. It has been designed to recognize P2RY6 from human, rat, and mouse samples.
For research purposes only, not for human use
Last Update: 29/10/2024
Applications
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