Overview
Alternative Name P2Y purinoceptor 6, P2RY6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)RNRTV(S)YDLSPPILS, corresponding to amino acid residues 172 - 186 of mouse P2RY6 (Accession Q9ERK9). 2nd extracellular loop.
Accession (Uniprot) Number Q9ERK9
Gene ID 233571
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
- Mouse and rat heart membrane lysates; mouse BV-2 microglia cell lysate and rat brain membranes; human MDA-MB-231 breast adenocarcinoma cell (1:200-1:2000).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) heart membranes:1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:400).
3,4. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).
Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).
3,4. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).
Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate:1. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).
2. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).
Immunohistochemistry
- Rat brain sections (1:1000).
Indirect flow cytometry
- Mouse J774 macrophage cells (2.5 µg).
Scientific background
The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily1,3.
P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels1,2.
P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries4.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
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