Name: Anti-P2Y6 Receptor (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: APR-106-F

Overview

Cat #: APR-106-F

Alternative Name P2Y purinoceptor 6, P2RY6

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RNRTV(S)YDLSPPILS, corresponding to amino acid residues 172-186 of mouse P2RY6 (Accession Q9ERK9). 2^nd extracellular loop.

Accession (Uniprot) Number Q9ERK9

Gene ID 233571

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Label Fluorescein isothiocyanate (FITC).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-106), and direct flow cytometry (labeled antibody).

Applications: fc, lci

May also work in: ic*

Direct flow cytometry

Cell surface detection of P2Y6 in live intact mouse BV-2 microglia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-P2Y6 Receptor (extracellular)-FITC Antibody (#APR-106-F), (2.5 µg).
Cell surface detection of P2Y6 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-P2Y6 Receptor (extracellular)-FITC Antibody (#APR-106-F), (2.5 µg).

References

Communi, D. et al. (2000) Cell Signal. 12, 351.
Filippov, A.K. et al. (1999) Br. J. Pharmacol. 126, 1009.
Chang, K. et al. (1995) J. Biol. Chem. 270, 26152.
Malmsjo, M. et al. (2003) B.M.C. Pharmacol. 3, 4.

Scientific background

The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily^1,3.

P2Y6 agonists induce an inositol phosphate/Ca²⁺ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca²⁺ and M-type K⁺ channels^1,2.

P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry applications. It has been designed to recognize P2RY6 from human, rat, and mouse samples.

Anti-P2Y6 Receptor (extracellular)-FITC Antibody (#APR-106-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Direct flow cytometry citations

Human neutrophils
Shulz, A. et al. (2020) Front. Immunol. 11, 582895.

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Reagent Rewards

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Navigation

Order Summary

Anti-P2Y6 Receptor (extracellular)-FITC Antibody