Name: Anti-PACAP Receptor 1 (PAC1) Antibody
Brand: Alomone Labs
SKU: AVR-003

Overview

Cat #: AVR-003

Alternative Name PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)KVNRYFTMDFKHRH, corresponding to amino acid residues 474-487 of rat PAC1 receptor (Accession P32215). Intracellular, C-terminus.

Accession (Uniprot) Number P32215

Gene ID 24167

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 12/14 amino acid residues identical.

RRID AB_2756805.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates:
1-3. Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003), (1:500).
4-6. Anti-PACAP Receptor 1 (PAC1) Antibody, preincubated with PACAP Receptor 1/PAC1 Blocking Peptide (#BLP-VR003).

Immunohistochemistry

Expression of PAC1 receptor in rat cerebellum
Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003), (1:400), followed by donkey anti-rabbit-Cy3 antibody. PAC1 receptor staining (red) appears in Purkinje cells (arrows). Nuclei are stained with DAPI (blue).

References

Shen, S. et al. (2013) Neuropeptides 47, 421.
Couvineau, A. and Laburthe, M. (2012) Br. J. Pharmacol. 166, 42.
Banki, E. et al. (2014) Neuropharmacology 85, 538.
Stevens, J.S. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, 3158.
Hattori, S. et al. (2012) Front. Behav. Neurosci. 6, 58.

Scientific background

Pituitary adenylate cyclase-activating polypeptide (PACAP, encoded by ADCYAP1) belongs a superfamily of structurally related peptide hormones, which include vasoactive intestinal peptide (VIP), glucagon, glucagon-like peptides, secretin, gastric inhibitory peptide and growth hormone-releasing hormone.

PACAP acts through PAC1, VPAC1 and VPAC2, initiating multiple signaling pathways¹. PAC1 is a class B member of the G-protein coupled receptors (GPCRs) superfamily. Class B receptors are differentiated from Class A by a relatively large N-terminal ectodomain that is crucial to ligand recognition².

PAC1R is mainly expressed on smooth muscle cells, neurons, endothelial cells and peritoneal macrophages³.

PAC1R is associated with posttraumatic stress disorder (PTSD) symptoms and psychophysiological markers of fear and anxiety⁴.

PAC1 receptor null mice demonstrate reduced anxiety-like behavior⁵. In addition, PAC1 overexpression can result in hydrocephalus- and schizophrenia-related neuropathology¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat PACAP type I receptor. Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PAC1 receptor from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

KO validation citations

Immunohistochemistry of rat nucleus accumbens shell. Tested in tissues treated with PACAP Receptor 1 shRNA.
Minnig, A.M. et al. (2021) Front. Behav. Neurosci. 15, 787362.

Specifications

Scientific Background

Specific Control Product

PACAP Receptor 1/PAC1 Blocking Peptide (#BLP-VR003) is the original antigen used for immunization during Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003) generation. The blocking peptide binds and ‘blocks’ Anti-PACAP Receptor 1/PAC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

PACAP Receptor 1/PAC1 Blocking Peptide (#BLP-VR003)

Shipping and Ordering Information

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Anti-PACAP Receptor 1 (PAC1) Antibody