Name: Anti-PACAP Receptor 1 (PAC1) Antibody
Brand: Alomone Labs
SKU: AVR-003

Overview

Cat #: AVR-003

Alternative Name PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)KVNRYFTMDFKHRH, corresponding to amino acid residues 474-487 of rat PAC1 receptor (Accession P32215). Intracellular, C-terminus.

Accession (Uniprot) Number P32215

Gene ID 24167

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 12/14 amino acid residues identical.

RRID AB_2756805.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Mouse and rat brain and rat cerebellum lysates (1:500-1:2500).
Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates:
1-3. Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003), (1:500).
4-6. Anti-PACAP Receptor 1 (PAC1) Antibody, preincubated with PACAP Receptor 1/PAC1 Blocking Peptide (#BLP-VR003).

Immunohistochemistry

Rat brain sections (1:400).

Scientific background

Pituitary adenylate cyclase-activating polypeptide (PACAP, encoded by ADCYAP1) belongs a superfamily of structurally related peptide hormones, which include vasoactive intestinal peptide (VIP), glucagon, glucagon-like peptides, secretin, gastric inhibitory peptide and growth hormone-releasing hormone.

PACAP acts through PAC1, VPAC1 and VPAC2, initiating multiple signaling pathways¹. PAC1 is a class B member of the G-protein coupled receptors (GPCRs) superfamily. Class B receptors are differentiated from Class A by a relatively large N-terminal ectodomain that is crucial to ligand recognition².

PAC1R is mainly expressed on smooth muscle cells, neurons, endothelial cells and peritoneal macrophages³.

PAC1R is associated with posttraumatic stress disorder (PTSD) symptoms and psychophysiological markers of fear and anxiety⁴.

PAC1 receptor null mice demonstrate reduced anxiety-like behavior⁵. In addition, PAC1 overexpression can result in hydrocephalus- and schizophrenia-related neuropathology¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

PACAP Receptor 1/PAC1 Blocking Peptide (#BLP-VR003) is the original antigen used for immunization during Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003) generation. The blocking peptide binds and ‘blocks’ Anti-PACAP Receptor 1/PAC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

PACAP Receptor 1/PAC1 Blocking Peptide (#BLP-VR003)

Shipping and Ordering Information

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Certificate of Analysis

Anti-PACAP Receptor 1 (PAC1) Antibody