Name: Anti-PACSIN1 Antibody
Brand: Alomone Labs
SKU: AIP-023

Overview

Cat #: AIP-023

Alternative Name Syndapin-1, Synaptic, Dynamin-associated protein I, Protein kinase C and casein kinase substrate in neurons protein 1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EWNPDLPHTAAKKEK corresponding to amino acid residues 301-315 of rat PACSIN1 (Accession Q9Z0W5). Intracellular.

Accession (Uniprot) Number Q9Z0W5

Gene ID 29704

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 14/15 amino acid residues identical; human – 13/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain (lanes 1 and 3), mouse brain (lanes 2 and 4), (1:200) and human brain neuroblastoma SH-SY5Y cell line lysates (lanes 5 and 6), (1:600):
1, 2, 5. Anti-PACSIN1 Antibody (#AIP-023).
3, 4, 6. Anti-PACSIN1 Antibody, preincubated with PACSIN1 Blocking Peptide (#BLP-IP023).

Immunohistochemistry

Expression of PACSIN1 in rat hippocampus.
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PACSIN1 Antibody (#AIP-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region, showed PACSIN1 immunoreactivity (green) in some neurons (arrows) in the pyramidal layer (P). B. Pre-incubation of the antibody with PACSIN1 Blocking Peptide (BLP-IP023), suppressed staining. Cell nuclei are stained with DAPI (blue).

References

Widagdo, J. et al. (2016) Sci. Rep. 6, 31070.
Kessels, M.M. and Qualmann, B. (2004) J. Cell Sci. 117, 3077.

Scientific background

The protein kinase C and casein kinase substrate in neurons (PACSIN), also known as syndapin, is an F-BAR (elongated BAR) and SH3 (src homology-3) domain-containing protein that is capable of remodeling the plasma membrane and mediating protein-protein interactions.

Three different PACSIN variants are identified, which are differentially expressed: PACSIN I is the neuronal‐specific isoform, PACSIN II is ubiquitously expressed and PACSIN III is expressed mainly in skeletal muscles and heart¹.

PACSINs interact with the large GTPase dynamin and several other proteins implicated in vesicle trafficking, PACSIN-dynamin complexes play an important role in vesicle fission at different donor membranes, including the plasma membrane (endocytosis) and Golgi membranes. In addition, PACSINs are implicated in later steps of vesicle cycling in neuronal and non-neuronal cells. PACSINs also engage in additional interactions with molecules involved in several signal transduction pathways, producing crosstalk at the interface between membrane trafficking and the cytoskeleton. Given the distinct expression patterns of the different PACSINs and their splice forms, these proteins could have isoform-specific functions².

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-PACSIN1 Antibody (#AIP-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize PACSIN1 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

PACSIN1 Blocking Peptide (#BLP-IP023) is the original antigen used for immunization during Anti-PACSIN1 Antibody (#AIP-023) generation. The blocking peptide binds and ‘blocks’ Anti-PACSIN1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

PACSIN1 Blocking Peptide (#BLP-IP023)

Shipping and Ordering Information

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Anti-PACSIN1 Antibody