Overview
- Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Western blot analysis of rat brain membranes:1. Anti-Pan NaV Antibody (#ASC-003), (1:200).
2. Anti-Pan NaV Antibody, preincubated with Pan Nav Blocking Peptide (#BLP-SC003).- Human atrial myofibroblasts (1:1000). (Chatelier, A. et al. (2012) J. Physiol. 590, 4307.).
- Rat brain (Anis, Y. et al. (1999) J. Biol. Chem. 274, 7431.).
Expression of NaV channels in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-Pan NaV Antibody (#ASC-003). A. NaV channels (red) appear in Purkinje cell bodies (vertical arrows) and in cells of the molecular layer (Mol), (horizontal arrows). B. Staining of Parvalbumin (green) in the same brain section. C. Confocal merge of NaV channels and Parvalbumin demonstrates the location of NaV channels to the cell body of Purkinje neurons.
- PC-3 human prostate epithelial tumor cell lines and Mat-LyLu rat epithelial tumor cell lines (Nakajima, T. et al. (2009) Br. J. Pharmacol. 156, 420.).
- Wu, L. et al. (2002) NeuroReport 13, 2547.
- Fang, X. et al. (2002) J. Neurosci. 22, 7425.
- Fjell, J. et al. (2000) NeuroReport 11, 199.
- Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
- Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
- Isom, L.L. (2001) Neuroscientist 7, 42.
- Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3
Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7
Alomone Labs is pleased to offer an antibody directed against an intracellular epitope of the rat NaV1.1 channel that is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels. Anti-Pan NaV Antibody (#ASC-003) can be used in western blot, immunocytochemistry, and immunohistochemistry applications.
Applications
Citations
Powered by Bioz- Rat brain neurolemma lysate.
Murenzi, E. et al. (2017) Neurotoxicology 60, 260. - Mouse neuron-derived CAD transfected cells.
Dustrude, E.T. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, E8443. - Mouse brain lysate (1:200).
Ban, Y. et al. (2015) J. Neurophysiol. 114, 520. - Mouse neuronal catecholamine A differentiated cells (CAD).
Dustrude, E.T. et al. (2013) J. Biol. Chem. 288, 24316. - Human atrial myofibroblasts (1:1000).
Chatelier, A. et al. (2012) J. Physiol. 590, 4307. - Rat brain.
Anis, Y. et al. (1999) J. Biol. Chem. 274, 7431. - Dog heart.
Yue, L. et al. (1999) Circ Res. 84, 776.
- Rat brain.
Anis, Y. et al. (1999) J. Biol. Chem. 274, 7431.
- Mouse cochleae sections.
Smith, K.E. et al. (2015) J. Neurosci. 35, 11221. - Guinea pig intestine.
Hanani, M. et al. (2000) Am J. Physiol. 278, G644.
- Rat hippocampus cell culture.
Sobieski, C. et al. (2015) J. Neurosci. 35, 11105. - Human PC3 prostate epithelial tumor cell line.
Nakajima, T. et al. (2009) Br. J. Pharmacol. 156, 420. - Rat Mat-LyLu epithelial tumor cell line.
Nakajima, T. et al. (2009) Br. J. Pharmacol. 156, 420.
