Name: Guinea pig Anti-Pan NaV Antibody
Brand: Alomone Labs
SKU: ASC-003-GP

Overview

Cat #: ASC-003-GP

Alternative Name Pan sodium channel, SP19

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: h, m, r

Immunogen

Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat Na_V1.1 (Accession P04774). Intracellular loop between domains III and IV.

Accession (Uniprot) Number P04774

Gene ID 81574

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology The epitope is identical in all isoforms of Na_V1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na⁺ channels.

RRID AB_2340959.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
1,2. Guinea pig Anti-Pan Na_VAntibody (#ASC-003-GP), (1:800).
3,4. Guinea pig Anti-Pan Na_V Antibody, preincubated with Pan Nav Blocking Peptide (#BLP-SC003).

References

Wu, L. et al. (2002) NeuroReport 13, 2547.
Fang, X. et al. (2002) J. Neurosci. 22, 7425.
Fjell, J. et al. (2000) NeuroReport 11, 199.
Baker, M.D. et al. (2001) Trends. Pharmacol. Sci. 22, 27.
Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
Isom, L.L. (2001) Neuroscientist 7, 42.
Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.

Scientific background

Voltage-gated sodium channels (Na_V) are essential for the generation of action potentials and for cell excitability.¹ Na_V channels are activated in response to depolarization and selectively allow flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-Na_V1.9.^4-5The Na_V channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (Na_V1.1, Na_V1.2, Na_V1.3, Na_V1.4, Na_V1.6 and Na_V1.7) and TTX-resistant (Na_V1.5, Na_V1.8 and Na_V1.9).^2-3

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β₁ through β₄ subunits, whereas sodium channels in adult skeletal muscle have only the β₁ subunit.^6,7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer an antibody directed against an epitope of the rat Na_V1.1 channel. The epitope is identical in all isoforms of Na_V1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na⁺ channels. Guinea pig Anti-Pan Na_V Antibody (#ASC-003-GP, formerly AGP-007), raised in guinea pigs, can be used in western blot analysis, and has been designed to recognize Na_V channels from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Pan Na_V Antibody (#ASC-003) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 20/11/2024

Applications

Citations

More product citations

Lee, C. et al. (2015) Biochem. Biophys. Res. Commun. 463, 1203.

Specifications

Scientific Background

Specific Control Product

Pan Nav Blocking Peptide (#BLP-SC003) is the original antigen used for immunization during Anti-Pan Na_V Antibody (#ASC-003) generation. The blocking peptide binds and ‘blocks’ Anti-Pan Nav primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Pan Nav Blocking Peptide (#BLP-SC003)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Guinea pig Anti-Pan NaV Antibody