Guinea pig Anti-Pan NaV Antibody

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Overview

Cat #: ASC-003-GP

Host: Guinea pig

Type: Polyclonal

Form: Lyophilized

Applications: WB

Reactivity: H, M, R

Alternative Name Pan sodium channel, SP19

Accession (Uniprot) Number P04774

Gene ID 81574

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

For research purposes only, not for human use

Specifications

Applications

Western blot

Rat brain and mouse brain membrane fractions (1:800-1:2000).
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
1,2. Guinea pig Anti-Pan Na_VAntibody (#ASC-003-GP), (1:800).
3,4. Guinea pig Anti-Pan Na_V Antibody, preincubated with Pan Nav Blocking Peptide (#BLP-SC003).
Following a broad screen of secondary antibodies, the following was used for this application: #106-035-006 (Jackson ImmunoResearch).

Scientific Background

Scientific background

Voltage-gated sodium channels (Na_V) are essential for the generation of action potentials and for cell excitability.¹ Na_V channels are activated in response to depolarization and selectively allow flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-Na_V1.9.^4-5The Na_V channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (Na_V1.1, Na_V1.2, Na_V1.3, Na_V1.4, Na_V1.6 and Na_V1.7) and TTX-resistant (Na_V1.5, Na_V1.8 and Na_V1.9).^2-3

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β₁ through β₄ subunits, whereas sodium channels in adult skeletal muscle have only the β₁ subunit.^6,7

Last Update: 25/03/2025

Blocking Peptide Control

Pan Nav Blocking Peptide (#BLP-SC003) is the original antigen used for immunization during Anti-Pan Na_V Antibody (#ASC-003) generation. The blocking peptide binds and ‘blocks’ Anti-Pan Nav primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Pan Nav Blocking Peptide (#BLP-SC003)