Name: Anti-PICK1 Antibody
Brand: Alomone Labs
SKU: APZ-014

Overview

Cat #: APZ-014

Alternative Name PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein interacting with PRKCA1, Protein kinase C-alpha-binding protein

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RARFSQMRKDVLEK, corresponding to amino acid residues 305-318 of rat PICK1 (Accession Q9EP80). Intracellular, α-helix domain.

Accession (Uniprot) Number Q9EP80

Gene ID 84591

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - identical.

RRID AB_2756773.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of human PANC-1 pancreatic carcinoma cell lysate (lanes 1 and 3) and human HepG2 liver carcinoma cell lysate (lanes 2 and 4):
1,2. Anti-PICK1 Antibody (#APZ-014), (1:200).
3,4. Anti-PICK1 Antibody, preincubated with PICK1 Blocking Peptide (#BLP-PZ014).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
1,2. Anti-PICK1 Antibody (#APZ-014), (1:200).
3,4. Anti-PICK1 Antibody, preincubated with PICK1 Blocking Peptide (#BLP-PZ014).

Immunohistochemistry

Expression of PICK1 in mouse hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PICK1 Antibody (#APZ-014), (1:400), followed by anti-rabbit-Cy3 antibody. PICK1 staining (red in the hippocampal CA3 region) is detected in neurons (arrows). Nuclei are stained with DAPI (blue).
Multiplex staining of GluR1 and PICK1 in rat hippocampus.
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004-GP), (1:400), followed by goat anti-guinea pig-AlexaFluor-488 and Anti-PICK1 Antibody (#APZ-014), (1:400), followed by donkey anti-rabbit-Cy3. A. GluR1 immunoreactivity (green) appears in several interneurons (arrows). B. PICK1 immunoreactivity (red) appears also in interneurons (arrows). C. Merge of the two images reveals co-localization in several interneurons (arrows) in the dentate gyrus (DG). Cell nuclei are stained with DAPI (blue).

References

Bolia, A. et al. (2012) Proteins 80, 1393.
Staudinger, J. et al. (1995) J. Cell Biol. 128, 263.
Zhao, B. et al. (2012) Cell Res. 22, 1467.
Zhang, B. et al. (2010) Cancer Sci. 101, 1536.
Jensen, L.J. et al. (2009) Nucleic Acids Res. 37, D412.

Scientific background

PICK1 interacts with the PDZ motifs of several proteins, including: protein kinase C α (PKC α), glutamate receptors (GluRs), prolactin-releasing peptide (PrRP), acid-sensing ion channel (ASICs), dopamine transporter (DAT), Karlirin 7, ephrin type-B receptor 2 (EphB2),ErbB2 / Her-2, TIS21, coxsackie virus and adenovirus receptor (CXADR), and UNC5H.

The proteins interacting with PICK1 have roles in synaptic plasticity, neuronal cell morphology, and mitochondrial-dependent apoptosis. In most of the cases, PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory¹.

PICK1 is widely expressed in the brain, testis, heart, lung, liver, kidney and muscle².

Its abnormal expression in brain has been linked to drug abuse and dependence, schizophrenia, and psychosis. Recent evidence suggest that PICK1 may affect human cancer development^3,4. PICK1 has also been reported to be expressed in insulin-producing pancreatic beta-cells where it may play a role in the neuroendocrine system⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat PICK1. Anti-PICK1 Antibody (#APZ-014) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Protein interacting with C kinase 1 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

PICK1 Blocking Peptide (#BLP-PZ014) is the original antigen used for immunization during Anti-PICK1 Antibody (#APZ-014) generation. The blocking peptide binds and ‘blocks’ Anti-PICK1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

PICK1 Blocking Peptide (#BLP-PZ014)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Anti-PICK1 Antibody