Name: Anti-PICK1 Antibody
Brand: Alomone Labs
SKU: APZ-014

Overview

Cat #: APZ-014

Alternative Name PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein interacting with PRKCA1, Protein kinase C-alpha-binding protein

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RARFSQMRKDVLEK, corresponding to amino acid residues 305-318 of rat PICK1 (Accession Q9EP80). Intracellular, α-helix domain.

Accession (Uniprot) Number Q9EP80

Gene ID 84591

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - identical.

RRID AB_2756773.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Human PANC-1 pancreatic carcinoma and HepG2 liver carcinoma cell lysates. Rat and mouse brain lysates (1:200-1:1000).
Western blot analysis of human PANC-1 pancreatic carcinoma cell lysate (lanes 1 and 3) and human HepG2 liver carcinoma cell lysate (lanes 2 and 4):
1,2. Anti-PICK1 Antibody (#APZ-014), (1:200).
3,4. Anti-PICK1 Antibody, preincubated with PICK1 Blocking Peptide (#BLP-PZ014).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
1,2. Anti-PICK1 Antibody (#APZ-014), (1:200).
3,4. Anti-PICK1 Antibody, preincubated with PICK1 Blocking Peptide (#BLP-PZ014).

Immunohistochemistry

Mouse brain sections (1:400).

Scientific background

PICK1 interacts with the PDZ motifs of several proteins, including: protein kinase C α (PKC α), glutamate receptors (GluRs), prolactin-releasing peptide (PrRP), acid-sensing ion channel (ASICs), dopamine transporter (DAT), Karlirin 7, ephrin type-B receptor 2 (EphB2),ErbB2 / Her-2, TIS21, coxsackie virus and adenovirus receptor (CXADR), and UNC5H.

The proteins interacting with PICK1 have roles in synaptic plasticity, neuronal cell morphology, and mitochondrial-dependent apoptosis. In most of the cases, PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory¹.

PICK1 is widely expressed in the brain, testis, heart, lung, liver, kidney and muscle².

Its abnormal expression in brain has been linked to drug abuse and dependence, schizophrenia, and psychosis. Recent evidence suggest that PICK1 may affect human cancer development^3,4. PICK1 has also been reported to be expressed in insulin-producing pancreatic beta-cells where it may play a role in the neuroendocrine system⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

PICK1 Blocking Peptide (#BLP-PZ014) is the original antigen used for immunization during Anti-PICK1 Antibody (#APZ-014) generation. The blocking peptide binds and ‘blocks’ Anti-PICK1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

PICK1 Blocking Peptide (#BLP-PZ014)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Reagent Rewards

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Navigation

Order Summary

Certificate of Analysis

Anti-PICK1 Antibody