Overview
- Peptide (C)EDLKPQHRRHISIR, corresponding to amino acid residues 1863-1876 of rat Piezo1 (Accession Q0KL00). The peptide is located in an internal region of the rat Piezo1 (the exact topology of Piezo1 remains to be defined).
Cell surface detection of Piezo1 by direct flow cytometry in live intact mouse M1 myeloid leukemia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-Piezo1 (extracellular)-FITC Antibody (#APC-087-F), (5µg).
Cell surface detection of Piezo1 by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-Piezo1 (extracellular)-FITC Antibody (#APC-087-F), (5µg).
- Gottlieb, P.A. and Sachs, F. (2012) Channels 6, 214.
- Faucherre, A. et al. (2014) Haematologica 99, 70.
- Coste, B. et al. (2010) Science 330, 55.
- Ranade, S.S. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, 10347.
Piezo1 protein is a family member of mixed cationic, non selective, directly mechanically activated ion channels found in higher eukaryotic cells. Piezo proteins are associated with physiological responses in cells that have been identified in many cell types and tissues in several species where mechanosensitivity plays a major role1,2.
Two mammalian Piezo proteins were identified, piezo1 and piezo2 encoded by the fam38A and fam38B genes recpectively2. Piezo1 expression is detected in organs such as: bladder, colon, kidney, lung and skin3.
The secondary structure and general length of Piezo proteins are quite conserved among species, and seem to form homotetrameric aggregates. Each subunit comprises between 24-32 putative transmembrane domains. The channels are constitutively active in liposomes and their activity does not require any other proteins1.
Mechanosensitive proteins have been linked to various pathophysiological situations such as hearing and deafness, cardiac physiological and hypertrophic responses, and kidney function and disease2.
Several studies have demonstrated that Piezo1 is essential for the removal of extra cells without apoptosis. Mutations in Piezo1 protein have been linked to the pathological response of red blood cells in a genetic disease called Xerocytosis. These finding suggest that Piezo1 plays a major role in cells’ responses to mechanical stimuli1. In addition, Piezo1 plays a critical role in endothelial cell mechanotransduction which is essential for embryonic development. Knock-out model of Piezo1 affects the ability of endothelial cells to alter their alignment when exposed to shear stress. Furthermore, loss of Piezo1 in endothelial cells leads to deficits in stress fiber and cellular orientation in response to shear stress, therefore Piezo1mechanotransduction is linked to regulation of cell morphology4.
Anti-Piezo1 (extracellular) Antibody (#APC-087) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize Piezo1 from mouse and rat samples. The antibody is will not recognize the protein from human samples
Anti-Piezo1 (extracellular)-FITC Antibody (#APC-087-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize Piezo1 from mouse and rat samples.
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