Name: Anti-Plexin-C1 (extracellular) Antibody
Brand: Alomone Labs
SKU: APR-091

Overview

Cat #: APR-091

Alternative Name Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EVKIQKENDNFNISK, corresponding to amino acid residues 860 - 874 of human Plexin-C1 (Accession O60486). Extracellular, N-terminus.

Accession (Uniprot) Number O60486

Gene ID 10154

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical. Rat – 14 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse brain membranes (lanes 1 and 4), rat brain membranes (lanes 2 and 5) and rat new born brain membranes (lanes 3 and 6):
1-3. Anti-Plexin-C1 (extracellular) Antibody (#APR-091), (1:200).
4-6. Anti-Plexin-C1 (extracellular) Antibody, preincubated with Plexin-C1 (extracellular) Blocking Peptide (BLP-PR091).
Western blot analysis of human HL-60 acute promyelocytic leukemia cell line lysates (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4):
1-2. Anti-Plexin-C1 (extracellular) Antibody (#APR-091), (1:200).
3-4. Anti-Plexin-C1 (extracellular) Antibody, preincubated with Plexin-C1 (extracellular) Blocking Peptide (BLP-PR091).

Immunohistochemistry

Expression of Plexin-C1 in in rat dorsal root ganglion.
Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-Plexin-C1 (extracellular) Antibody (#APR-091), (1: 200), followed by goat anti-rabbit-AlexaFluor-488. A. Plexin-C1 immunoreactivity (green) appears in DRG neuron profiles (arrows). B. Pre-incubation of the antibody with Plexin-C1 (extracellular) Blocking Peptide (BLP-PR091), suppressed staining. Cell nuclei are stained with DAPI (blue).

Indirect flow cytometry

Cell surface detection of Plexin-C1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Plexin-C1 (extracellular) Antibody (#APR-091), (2.5μg) + goat-anti-rabbit-FITC.

References

Perälä. et al. (2012), Differentiation, 83, 77.
Chen. et al. (2012), Oncogene, 32, 4941.
Scott. et al. (2009), J Invest Dermatol, 129, 954.
König. et al. (2014), Eur J Immunol. 44, 2648.

Scientific background

Plexin-C1 also known as Virus-encoded semaphorin protein receptor, is a ∼210 kDa type I transmembrane receptor that regulates cytoskeletal remodeling and adhesion in human melanocytes.

It belongs to the family of Plexin receptors, these are type I transmembrane proteins (A–D) that share a highly conserved cytoplasmic domain and an intracellular region containing an intrinsic Ras-GTPase activating (GAP) domain. Plexin receptors bind semaphorins: a class of secreted and membrane proteins ¹. The natural ligand of Plexin C1 is Semaphorin -7A (#ASR-077).

Plexin-C1 is highly expressed in melanocytes, neurons, neutrophils, dendritic cells and pre-osteoblasts and it has a complex effect on migration of melanocytes and melanoma, that depends on the phase of progression of this type of cancer ^2,3. It also has additional roles in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, in the development of inflamation and in inhibition of neutrophils phagocytosis ^3,4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Plexin-C1 (extracellular) Antibody (#APR-091) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize PLXNC1 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

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Anti-Plexin-C1 (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

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