Name: Anti-Plexin-C1 (extracellular) Antibody
Brand: Alomone Labs
SKU: APR-091

Back to product page SDS

Overview

Cat #: APR-091

Alternative Name Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EVKIQKENDNFNISK, corresponding to amino acid residues 860 - 874 of human Plexin-C1 (Accession O60486). Extracellular, N-terminus.

Accession (Uniprot) Number O60486

Gene ID 10154

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical. Rat – 14 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse brain membranes (lanes 1 and 4), rat brain membranes (lanes 2 and 5) and rat new born brain membranes (lanes 3 and 6):
1-3. Anti-Plexin-C1 (extracellular) Antibody (#APR-091), (1:200).
4-6. Anti-Plexin-C1 (extracellular) Antibody, preincubated with Plexin-C1 (extracellular) Blocking Peptide (BLP-PR091).
Western blot analysis of human HL-60 acute promyelocytic leukemia cell line lysates (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4):
1-2. Anti-Plexin-C1 (extracellular) Antibody (#APR-091), (1:200).
3-4. Anti-Plexin-C1 (extracellular) Antibody, preincubated with Plexin-C1 (extracellular) Blocking Peptide (BLP-PR091).

Scientific background

Plexin-C1 also known as Virus-encoded semaphorin protein receptor, is a ∼210 kDa type I transmembrane receptor that regulates cytoskeletal remodeling and adhesion in human melanocytes.

It belongs to the family of Plexin receptors, these are type I transmembrane proteins (A–D) that share a highly conserved cytoplasmic domain and an intracellular region containing an intrinsic Ras-GTPase activating (GAP) domain. Plexin receptors bind semaphorins: a class of secreted and membrane proteins ¹. The natural ligand of Plexin C1 is Semaphorin -7A (#ASR-077).

Plexin-C1 is highly expressed in melanocytes, neurons, neutrophils, dendritic cells and pre-osteoblasts and it has a complex effect on migration of melanocytes and melanoma, that depends on the phase of progression of this type of cancer ^2,3. It also has additional roles in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, in the development of inflamation and in inhibition of neutrophils phagocytosis ^3,4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Navigation

Order Summary

Certificate of Analysis

Anti-Plexin-C1 (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

Need Help With This Product?