Overview
- Peptide (C)EVKIQKENDNFNISK, corresponding to amino acid residues 860 - 874 of human Plexin-C1 (Accession O60486). Extracellular, N-terminus.
Cell surface detection of Plexin-C1 by direct flow cytometry in live intact mouse J774 macrophage cell line:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Plexin-C1 (extracellular)-FITC Antibody (#APR-091-F), (2.5µg).
Cell surface detection of Plexin-C1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Plexin-C1 (extracellular)-FITC Antibody (#APR-091-F), (5µg).
- Perälä. et al. (2012), Differentiation, 83, 77.
- Chen. et al. (2012), Oncogene, 32, 4941.
- Scott. et al. (2009), J Invest Dermatol, 129, 954.
- König. et al. (2014), Eur J Immunol. 44, 2648.
Plexin-C1 also known as Virus-encoded semaphorin protein receptor, is a ∼210 kDa type I transmembrane receptor that regulates cytoskeletal remodeling and adhesion in human melanocytes.
It belongs to the family of Plexin receptors, these are type I transmembrane proteins (A–D) that share a highly conserved cytoplasmic domain and an intracellular region containing an intrinsic Ras-GTPase activating (GAP) domain. Plexin receptors bind semaphorins: a class of secreted and membrane proteins 1. The natural ligand of Plexin C1 is Semaphorin -7A (#ASR-077).
Plexin-C1 is highly expressed in melanocytes, neurons, neutrophils, dendritic cells and pre-osteoblasts and it has a complex effect on migration of melanocytes and melanoma, that depends on the phase of progression of this type of cancer 2,3. It also has additional roles in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, in the development of inflamation and in inhibition of neutrophils phagocytosis 3,4.
Anti-Plexin-C1 (extracellular) Antibody (#APR-091) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize PLXNC1 from mouse, rat and human samples.
Anti-Plexin-C1 (extracellular)-FITC Antibody (#APR-091-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize PLXNC1 from mouse, rat and human samples.
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