Overview
- Peptide (C)GVYMRFDVPRPRVKR, corresponding to amino acid residues 315 - 329 of mouse SLC29A4 (Accession Q8R139). Intracellular, 3rd loop.
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1-2. Anti-PMAT (SLC29A4) Antibody (#ANT-054), (1:400).
3-4. Anti-PMAT (SLC29A4) Antibody, preincubated with PMAT (SLC29A4) Blocking Peptide (BLP-NT054). - Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human Caco2 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):1-2. Anti-PMAT (SLC29A4) Antibody (#ANT-054), (1:400).
3-4. Anti-PMAT (SLC29A4) Antibody, preincubated with PMAT (SLC29A4) Blocking Peptide (BLP-NT054).
- Expression of PMAT in rat supraoptic nucleus (SON).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PMAT (SLC29A4) Antibody (#ANT-054), (1:1000), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. PMAT immunoreactivity (red) appears in SON neurons (arrows). B. Pre-incubation of the antibody with PMAT (SLC29A4) Blocking Peptide (BLP-NT054), suppressed staining. Cell nuclei are stained with DAPI (blue). Abbreviations: OT = optic tract, SON = supraoptic nucleus.
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Plasma membrane monoamine transporters (PMAT) is a monoamine transporter which has functional and structural similarities to the organic cation transporters (OCT) family1,2. PMAT first cloned in 2004 from the human brain. PMAT transporter belongs to the equilibrative nucleoside transporter (ENT) family but it functions as a polyspecific organic cation transporter3. PMAT (ENT4) has a low-affinity and high-capacity for monoamines 3,4. It was identified as a sodium-independent, electrogenic cation transporter with a wide range of substrates. The protein contains 530 amino acids with 11 transmembrane domains, very similar to the organic cation transporters (OCT) family. A peculiarity of this transporter is its ability to function bidirectionally 1,2,4. PMAT is highly expressed in the brain and skeletal muscle and also found in the liver, kidney, intestine and heart. It efficiently transports many organic cations such as 1-methyl-4-phenylpyridinium (MPP+), which is also the case for OCTs. In particular, PMAT transports histamine with a low affinity but efficiently (high Vmax) 4,5. Similarly, both PMAT and OCTs are sensitive to decynium-22 blockade 4,6. However, PMAT was insensitive to the steroid corticosterone, unlike OCTs 3. Direct comparison of transport efficiency (Vmax/Km) between human OCT3 and PMAT expressed in HEK cells found that 5-HT and dopamine were preferentially taken up by PMAT. Alternatively, OCT3 has a higher preference for histamine, noradrenaline and adrenaline over other biogenic amines 7. The same study found that PMAT was insensitive to inhibitors of high-affinity monoamine transporters such as citalopram, GBR-12909 and desipramine. An earlier study substantiated this by showing a range of antidepressant and antipsychotic drugs had IC50’s of 10-20µM at the human PMAT8. PMAT distribution has been investigated in mouse and rat brain9,10. PMAT reported to be widely distributed throughout the brain and not necessarily associated with monoamine pathways. Indeed, it was completely absent from the rat substantia nigra. Expression of PMAT has been reported to be primarily neuronal. Cells double labeled with glial fibrillary acidic protein and PMAT were not observed in any great number in the mouse brain9. However, there is evidence PMAT expression in cultured human astrocytes where they mediate histamine transport11.
Application key:
Species reactivity key:
Anti-PMAT (SLC29A4) Antibody (#ANT-054) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMAT from rat, mouse and human samples.