Name: Anti-PMCA2 Antibody
Brand: Alomone Labs
SKU: ACP-002

Overview

Cat #: ACP-002

Alternative Name Plasma membrane Ca²⁺ ATPase 2, ATP2B2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)KEIPDPSSINAKTLE, corresponding to amino acid residues 522-536 of rat PMCA2 (Accession P11506). 2^nd intracellular loop.

Accession (Uniprot) Number P11506

Gene ID 24215

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 13/15 amino acid residues identical.

RRID AB_2340922.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN_3.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
1,2. Anti-PMCA2 Antibody (#ACP-002), (1:400).
3,4. Anti-PMCA2 Antibody, preincubated with PMCA2 Blocking Peptide (#BLP-CP002).

References

Jensen, T.P. et al. (2004) Brain Res. Dev. Brain Res. 152, 129.
Strehler, E.E. and Zacharias, D.A. (2001) Physiol. Rev. 81, 21.
Talarico, E.F. et al. (2005) Mol. Vis. 11, 169.
Jensen, T.P. et al. (2007) J. Physiol. 579, 85.
Burette, A. and Weinberg, R.J. (2007) J. Comp. Neurol. 500, 1127.

Scientific background

The plasma membrane calcium ATPase (PMCA) is a transport protein in the plasma membrane of cells responsible for removing calcium (Ca²⁺) from the cell. This pump is vital for regulating the amount of Ca²⁺ within cells¹.

The PMCA and the sodium/calcium exchanger (NCX) are together the main regulators of intracellular Ca²⁺ concentrations².

Since it transports Ca²⁺ into the extracellular space, the PMCA is also an important regulator of the Ca²⁺ concentration in the extracellular space³.

The PMCA belongs to a family of P-type primary ion transport ATPases, and is expressed in a variety of tissues, including the brain⁴.

The pump is powered by the hydrolysis of adenosine triphosphate (ATP), with a stoichiometry of one Ca²⁺ ion removed for each molecule of ATP hydrolyzed. It binds to Ca²⁺ ions with a high affinity (a Km of 100 to 200 nM) but does not remove Ca²⁺ at a very fast rate. This is in contrast to the NCX, which has a low affinity and a high capacity. Thus, the PMCA is effective at binding Ca²⁺ even when its concentration within the cell is very low, and so is suited to maintaining Ca²⁺ at its normally very low levels. The NCX is better suited to removing large amounts of Ca²⁺ quickly, as it is needed in neurons after an action potential. Thus the activities of the two types of pump complement each other⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat PMCA2. Anti-PMCA2 Antibody (#ACP-002) can be used in western blot analysis and has been designed to recognize PMCA2 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

PMCA2 Blocking Peptide (#BLP-CP002) is the original antigen used for immunization during Anti-PMCA2 Antibody (#ACP-002) generation. The blocking peptide binds and ‘blocks’ Anti-PMCA2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

PMCA2 Blocking Peptide (#BLP-CP002)

Shipping and Ordering Information

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Anti-PMCA2 Antibody