Name: Anti-PMCA3 Antibody
Brand: Alomone Labs
SKU: ACP-003

Overview

Cat #: ACP-003

Alternative Name Plasma membrane Ca²⁺ ATPase 3, Plasma membrane calcium pump isoform 3, ATP2B3, SCAX1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)EFHPKPQ(N)QREVPH, corresponding to amino acid residues 10-23 of rat PMCA3 (Accession Q64568). Intracellular, N-terminus.

Accession (Uniprot) Number Q64568

Gene ID 29599

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse – 13/14 amino acid residues identical; human – 11/14 amino acid residues identical.

RRID AB_2756565.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
1,2. Anti-PMCA3 Antibody (#ACP-003), (1:200).
3,4. Anti-PMCA3 Antibody, preincubated with PMCA3 Blocking Peptide (#BLP-CP003).

Immunohistochemistry

Expression of PMCA3 in rat choroid plexus
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PMCA3 Antibody (#ACP-003), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. PMCA3 immunoreactivity (green) appears in the outlines of the choroid plexus (arrows) located in the lateral ventricle of the brain. Cell nuclei are stained with DAPI (blue).

References

Padányi, R. et al. (2016) Biochim. Biophys. Acta 1863, 1351.
Monesterolo, N.E. et al. (2015) Biochim Biophys Acta 1848, 2813.
Delgado-Coello, B. et al. (2014) Organogenesis 10, 333.

Scientific background

PMCAs (plasma membrane calcium ATPases) are P-type ATPases, a separate sub-group of Ca^2 + pumps responsible for maintain intracellular Ca²⁺ homeostasis. PMCAs reduce Ca^2 + in the cytosol by direct ejection, by controlling the formation of inositol-1,4,5-trisphosphate (IP₃), and by decreasing Ca^2 + release from the endoplasmic reticulum Ca²⁺ pool^1,2.

Four PMAC proteins are expressed in mammals: PMCA1–4. Alternative splicing produces more than twenty variants that are dissimilar in their regulatory characteristics^1,3. PMCA pumps have a C-terminus with a high affinity calmodulin-binding site that also acts as an auto-inhibitor. The C-terminal region also has phosphorylation sites as well as proteolytic sites for caspase-3 and calpain and several motifs that control trafficking of the pump^1,2.

PMCA3 is usually present in excitable cells, in the brain and in hair cells of the inner ear or skeletal muscle. The variant is quickly activated and stays active for a long period of time¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-PMCA3 Antibody (#ACP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA3 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

PMCA3 Blocking Peptide (#BLP-CP003) is the original antigen used for immunization during Anti-PMCA3 Antibody (#ACP-003) generation. The blocking peptide binds and ‘blocks’ Anti-PMCA3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

PMCA3 Blocking Peptide (#BLP-CP003)

Shipping and Ordering Information

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Anti-PMCA3 Antibody