Overview
- Peptide (C)PEDYPDQFDDVMD, corresponding to amino acid residues 54-66 of human GDNF (precursor) (Accession P39905). Pro-domain of the GDNF protein.
Western blot analysis of human GDNF pro-domain fusion protein at 40 ng (lanes 1 and 4), 30 ng (lanes 2 and 5) and 20ng (lanes 3 and 6):1-3. Anti-proGDNF Antibody (#ANT-026), (1:200).
4-6. Anti-proGDNF Antibody, preincubated with proGDNF Blocking Peptide (#BLP-NT026).
Note that the expected MW of the GDNF pro-domain fusion protein is 21kD.
- Airaksinen, M.S. and Saarma, M. (2002) Nat. Rev. Neurosci. 3, 383.
- Henderson, C.E. et al. (1994) Science 266, 1062.
- Houenou, L.J. et al. (1996) Cell. Tissue Res. 286, 219.
- Kobayashi, M. et al. (2000) Neuroreport 11, 2541.
- Tomac, A. et al. (1995) Nature 373, 335.
- Zhou, F.Q. et al. (2003) Cell 113, 814.
- Lin, L.F. et al. (1993) Science 260, 1130.
- Matheson, C.R. et al. (1997) Neuroreport 8, 1739.
- Brundin, P. (2002) Brain 125, 2149.
- Grondin, R. et al. (1998) J. Neurol. 245, P35.
- Bradley, L.H. et al. (2010) PLoS ONE 5, e9752.
- Immonen, T. et al. (2008) Exp. Neurol. 210, 793.
- Kelps, K.A. et al. (2011) Neuropeptides 45, 213.
Glial-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily. GDNF signals through a multi-component receptor system, composed of a RET protooncogene and one of the four α1-α4 receptors1.
GDNF promotes survival of various neuronal cells, including motoneurons2,3, Purkinje cells and sympathetic neurons4. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake5. Cells that express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells6, neurons, pinealocytes, and skeletal muscle cells7.
In vivo, following transection of facial motor neuron axons, locally applied GDNF has been shown to rescue virtually all damaged neurons from death8. GDNF may be of clinical relevance in the treatment of Parkinson's disease that is characterized by progressive degeneration of midbrain dopaminergic neurons9,10.
Recently, it has been hypothesized that functional, carboxy-terminally amidated peptides are processed from the GDNF precursor upon proteolytic cleavage by furin-like endopeptidase11,12,13. Those different peptides (a 5-mer and 11-mer) have not been isolated endogenously to date. However, the rat 11-mer sequence (named brain excitatory peptide, BEP) significantly induced synaptic excitability and possessed some dopaminergic activities in vitro (thus named dopamine neuron stimulating peptides, DNSP)13. Furthermore, the human 11-mer sequence (named DNSP-11) exhibits neurotrophic-like properties13.
Thus, the role of the full proDomain of GDNF, which is a product of proteolytic cleavage of proGDNF, is not clearly understood yet.
Anti-proGDNF Antibody (#ANT-026) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize proGDNF from human, mouse, and rat samples. The antibody is specific for the pro-domain region of GDNF and will not react with mature GDNF.
Powered by Bioz
