Overview
- Peptide (C)ENTTNTFTDFFSARD, corresponding to amino acid residues 10-24 of rat PROKR1 (Accession Q8R416). Extracellular, N-terminus.
Western blot analysis of rat brain membranes:1. Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:200).
2. Anti-Prokineticin Receptor 1 (extracellular) Antibody, preincubated with Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041).
Western blot analysis of mouse brain lysate:1. Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:200).
2. Anti-Prokineticin Receptor 1 (extracellular) Antibody, preincubated with Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041).
Expression of Prokineticin receptor 1 in rat DRGImmunohistochemical staining of frozen rat dorsal root ganglion (DRG) sections using Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:100), followed by Alexa-555-conjugated goat anti-rabbit secondary antibody. PK-R1 (red) is expressed in DRG neurons. Cell nuclei are visualized with Hoechst 33342 (blue).
Expression of Prokineticin receptor 1 in mouse brainImmunohistochemical staining of mouse olfactory bulb brain sections using Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:100). A. Green fluorescence reveals intensely stained astrocyte-like cells in the glomeruli (G) and lightly stained astrocyte-like cells in the adjacent layer (arrows). B. Merge of images of PK-R1 (green), parvalbumin (red, a neuronal marker) and DAPI (blue) nuclear counterstain.
Cell surface detection of Prokineticin receptor 1 by indirect flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (2.5μg) + goat-anti-rabbit-FITC.- The blocking peptide is not suitable for this application.
- Zhou, Q.Y. (2006) Mol. Interv. 6, 330.
- Negri, L. et al. (2006) J. Neurosci. 26, 6716.
- Dorsch, M. et al. (2005) J. Leucoc. Biol. 78, 426.
The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.
The biological effects of PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin receptor 1 (PKR1) and Prokineticin receptor 2 (PKR2) that belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1
PKR1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization. In addition, activation of the mitogen-activated protein kinase (MAPK) pathways has also been described.1
PKR1 is broadly distributed throughout peripheral tissues including the intestinal tract, testis, uterus, lung and peripheral blood leukocytes. In addition, the receptor is expressed in the brain, particularly in olfactory regions, as well as in dorsal root ganglion (DRG) neurons.
The physiological function of PKR1 is still being elucidated, however, evidence suggest that the receptor has an important role in pain perception through its ability to stimulate the TRPV1 ion channel in DRG neurons.2
In addition, a role for PKR1 in the control of the inflammatory response through regulation of macrophage chemotaxis and cytokine production has also been demonstrated.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminal domain of the rat Prokineticin receptor 1. The epitope is specific for PROKR1 and will not cross-react with the closely related PROKR2. Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041) can be used in western blot, immunohistochemistry and flow cytometry applications, and recognizes PROKR1 from rat and mouse samples. The antibody will not recognize the receptor from human samples.
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