Name: Anti-Prokineticin Receptor 1 (extracellular) Antibody
Brand: Alomone Labs
SKU: APR-041

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Overview

Cat #: APR-041

Alternative Name PROKR1, PKR1, G-protein coupled receptor 73, Gpr73, Gpr73a, G-protein coupled receptor ZAQ

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)ENTTNTFTDFFSARD, corresponding to amino acid residues 10-24 of rat PROKR1 (Accession Q8R416). Extracellular, N-terminus.

Accession (Uniprot) Number Q8R416

Gene ID 192648

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 12/15 amino acid residues identical.

RRID AB_2039853.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Unlikely to recognize human samples.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Rat and mouse brain samples (1:200).
Western blot analysis of rat brain membranes:
1. Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:200).
2. Anti-Prokineticin Receptor 1 (extracellular) Antibody, preincubated with Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041).
Western blot analysis of mouse brain lysate:
1. Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:200).
2. Anti-Prokineticin Receptor 1 (extracellular) Antibody, preincubated with Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041).

Immunohistochemistry

Perfusion fixed, frozen sections of rat dorsal root ganglion (DRG) (1:100) and mouse brain sections (1:100).

Indirect flow cytometry

WEHI-231 (mouse B cell lymphoma) cell lines (1:20).
The blocking peptide is not suitable for this application.

Scientific background

The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.

The biological effects of PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin receptor 1 (PKR1) and Prokineticin receptor 2 (PKR2) that belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.¹

PKR1 receptors couple to G_q/G₁₁ proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization. In addition, activation of the mitogen-activated protein kinase (MAPK) pathways has also been described.¹

PKR1 is broadly distributed throughout peripheral tissues including the intestinal tract, testis, uterus, lung and peripheral blood leukocytes. In addition, the receptor is expressed in the brain, particularly in olfactory regions, as well as in dorsal root ganglion (DRG) neurons.

The physiological function of PKR1 is still being elucidated, however, evidence suggest that the receptor has an important role in pain perception through its ability to stimulate the TRPV1 ion channel in DRG neurons.²

In addition, a role for PKR1 in the control of the inflammatory response through regulation of macrophage chemotaxis and cytokine production has also been demonstrated.³

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041) is the original antigen used for immunization during Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041) generation. The blocking peptide binds and ‘blocks’ Anti-Prokineticin Receptor 1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041)

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Anti-Prokineticin Receptor 1 (extracellular) Antibody