Name: Anti-proNT-3 Antibody
Brand: Alomone Labs
SKU: ANT-012

Back to product page SDS

Overview

Cat #: ANT-012

Alternative Name pro-Neurotrophin 3, proNTF3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor) (Accession P20783). Pro-domain of the NT-3 protein.

Accession (Uniprot) Number P20783

Gene ID 4908

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse - identical.

RRID AB_2040015.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity The antibody is specific for proNT-3, it does not crossreact with proNGF, proBDNF or mature NT-3.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Recombinant mouse proNT-3.
Western blot analysis using Anti-proNT-3 Antibody (#ANT-012) (1:600):
1. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (200 ng).
2. Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (200 ng).
3. Native mouse NGF 2.5S protein (>95%) (#N-100), (200 ng).
4. Recombinant human beta-NGF protein (#N-245), (200 ng).
5. Recombinant human BDNF protein (#B-250), (200 ng).
6. Recombinant mouse proNGF protein (#N-250), (200 ng).
7. Recombinant mouse proBDNF protein (#B-240), (200 ng).
8. Recombinant proNT-3, (10 ng).
Western blot analysis of mouse recombinant proNT-3:
1,5. 10 ng proNT-3.
2,6. 50 ng proNT-3.
3,7. 100 ng proNT-3.
4,8. 200 ng proNT-3.
Lanes 1-4 Anti-proNT-3 Antibody (#ANT-012), (1:300).
Lanes 5-8 Anti-proNT-3 Antibody, preincubated with proNT-3 Blocking Peptide (#BLP-NT012).

Immunohistochemistry

Mouse brain sections.

Scientific background

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).

All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins. These are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.

Until recently, the functional role of the neurotrophin prodomain was thought to assist in the correct folding of the mature protein and the sorting of the neurotrophin into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions. Several studies implicate both proNGF and proBDNF in several physiological and pathological conditions.

It remains to be established whether proNT-3 has different biological functions than the mature NT-3 neurotrophin.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

proNT-3 Blocking Peptide (#BLP-NT012) is the original antigen used for immunization during Anti-proNT-3 Antibody (#ANT-012) generation. The blocking peptide binds and ‘blocks’ Anti-proNT-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

proNT-3 Blocking Peptide (#BLP-NT012)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Reagent Rewards

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Navigation

Order Summary

Certificate of Analysis

Anti-proNT-3 Antibody