Anti-Prostanoid EP1 Receptor (ext.)-FITC Antibody

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Overview

Cat #: APR-063-F Size: 50 µl Lot: APR063FAN0150

Alternative Name Prostaglandin E2 receptor EP1 subtype, PGE2 receptor EP1 subtype, Prostanoid EP1 receptor, PTGEREP1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)GPDDRRRWGSRGPR, corresponding to amino acid residues 249-262 of mouse prostanoid EP1 receptor (Accession P35375). 3^rd extracellular loop.

Accession (Uniprot) Number P35375

Gene ID 19216

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label Fluorescein isothiocyanate (FITC).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-063), and direct flow cytometry (labeled antibody).

Applications: fc

May also work in: ic*, lci*

Direct flow cytometry

Human THP-1 monocytic leukemia and mouse J774 macrophage cells (2.5 µg).
Cell surface detection of PTGER1 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular)-FITC Antibody (#APR-063-F), (2.5 µg).
Cell surface detection of PTGER1 in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular)-FITC Antibody (#APR-063-F), (2.5 µg).

Scientific background

Prostaglandin (PG) E2 is involved in a number of physiological and pathophysiological events in many tissues throughout the body. The biological effects of PGE2 are mediated through interaction with specific membrane-bound G-protein coupled prostanoid EP receptors. There are four subtypes of the EP receptor, termed EP1, EP2, EP3 and EP4, which have been defined on the basis of their different pharmacological profiles and signal transduction pathways¹.

EP1 is characterized by the presence of a helical bundle consisting of seven hydrophobic transmembrane helices (TM1-TM7), which are connected through six alternating extracellular and intracellular loops. The N-terminus is located on the extracellular side of the membrane, whereas the C-terminus is located on the intracellular side².

EP1 receptors are thought to mediate contraction of smooth muscle in various tissues such as the gastrointestinal tract, respiratory tract, vas deferens, myometrium and the iris sphincter muscle and may also be important for absorption inhibition in the small intestine as well as enhancement of neuronal activity³.

Human colon cancer cells express the EP1 receptor. Furthermore, EP1 receptor antagonists inhibit chemically induced breast cancer development in rats and reduce the number of skin tumors per mouse following UVB exposure^4,5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 04/07/2019

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Scientific Background

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