Name: Anti-PTGFRN (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-212
Rating: 4.6 (2 reviews)

Overview

Cat #: ANR-212

Alternative Name Prostaglandin F2 Receptor Inhibitor, CD9 Partner 1, CD9P-1, FPRP, Glu-Trp-Ile EWI motif-containing protein F, EWI-F, CD315

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EDEFRYRMYQTQVSD, corresponding to amino acid residues 635 - 649 of mouse PTGFRN (Accession Q9WV91). Extracellular, N-terminus.

Accession (Uniprot) Number Q9WV91

Gene ID 19221

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical. Human – 14 out of 15 amino acid identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 2 µg peptide per 1 µg antibody
PTGFRN (extracellular) Blocking Peptide (#BLP-NR212)

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
1-2. Anti-PTGFRN (extracellular) Antibody (#ANR-212), (1:400).
3-4. Anti-PTGFRN (extracellular) Antibody, preincubated with PTGFRN (extracellular) Blocking Peptide (BLP-NR212).
Western blot analysis of human pancreatic carcinoma PANC-1 cell line lysate (lanes 1 and 4), human breast adenocarcinoma MCF-7 cell line (lanes 2 and 5) and human U-87 MG glioblastoma cell line (lanes 3 and 6):
1-3. Anti-PTGFRN (extracellular) Antibody (#ANR-212), (1:400).
4-6. Anti-PTGFRN (extracellular) Antibody, preincubated with PTGFRN (extracellular) Blocking Peptide (BLP-NR212).

Immunohistochemistry

Expression of PTGFRN in mouse hypothalamus:
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-PTGFRN (extracellular) Antibody (#ANR-212), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. PTGFRN immunoreactivity (green) appears in cells of ventromedial hypothalamus (arrows). B. Pre-incubation of the antibody with PTGFRN (extracellular) Blocking Peptide (BLP-NR212), suppressed staining. Cell nuclei are stained with DAPI (blue). 3^rd V = 3^rd ventricle.
Expression of PTGFRN in rat hippocampal dentate gyrus:
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PTGFRN (extracellular) Antibody (#ANR-212), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. PTGFRN immunoreactivity (green) appears in cells of the sub-granular layer (GL). B. Pre-incubation of the antibody with PTGFRN (extracellular) Blocking Peptide (BLP-NR212), suppressed staining. Cell nuclei are stained with DAPI (blue).

Indirect flow cytometry

Cell surface detection of PTGFRN by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-PTGFRN (extracellular) Antibody (#ANR-212), (2.5μg) + goat-anti-rabbit-APC.

References

Mala et al. (2022) BMC Cancer, 22, 642.
Aguila et al. (2019) Cancer Lett., 462, 33–42.
Muraoka et al. (2020) Cells, 9, 1959.
Marquez et al. (2021) PLoS One, 16, e0246197.

Scientific background

The Prostaglandin F2 Receptor Negative Regulator (PTGFRN), also known as EWI-F and CD9P-1, is a member of the immunoglobulin (Ig) superfamily. PTGFRN is a type I single-pass transmembrane protein and part of the cell adhesion molecule (CAM) family, which plays essential roles in cell-cell and cell-matrix interactions^1,2.

CAMs, including PTGFRN, are characterized by extracellular Ig-like domains and contribute to cellular adhesion, migration, and signaling. PTGFRN interacts with tetraspanins (CD9, CD81), integrins, and ERM proteins, functioning as a scaffold in tetraspanin-enriched microdomains (TEMs)³. These interactions enable its role in tumor progression, metastasis, and angiogenesis by regulating PI3K/AKT and mTOR signaling pathways^1,2.

PTGFRN is highly expressed in glioblastoma (GBM) and several cancers, with significantly elevated levels in tumor tissues compared to normal tissues^1,2,3. It is implicated in tumor proliferation, migration, and radiation resistance. In neurodegenerative disorders, PTGFRN correlates with Alzheimer’s disease progression via its role in extracellular vesicles (EVs) associated with amyloid plaques³.

PTGFRN’s association with poor prognosis in cancers and its involvement in neurodegeneration highlight its potential as a therapeutic target. Immunodetection of PTGFRN can benefit research areas in oncology, neurobiology, and regenerative medicine^1,2,4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-PTGFRN (extracellular) Antibody (#ANR-212) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize PTGFRN from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 19/12/2024

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Anti-PTGFRN (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

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