Name: Anti-RAMP2 (extracellular) Antibody
Brand: Alomone Labs
SKU: ARR-022

Overview

Cat #: ARR-022

Alternative Name Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)HPTEDSLLSKGKMED, corresponding to amino acid residues 54-68 of rat RAMP2 (Accession Q9JHJ1). Extracellular, N-terminus.

Accession (Uniprot) Number Q9JHJ1

Gene ID 58966

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 14/15 amino acid residues identical.

RRID AB_2756789.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Not recommended for use with human samples.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Rat lung, spleen, cortex, and aortic endothelial cell lysates (RAEC). Mouse heart lysate (1:200-1:1000).
Western blot analysis of rat lung (lanes 1 and 4, 1:200), mouse heart (lanes 2 and 5, 1:200) and rat aortic endothelial cell (RAEC) (lanes 3 and 6, 1:400) lysates:
1-3. Anti-RAMP2 (extracellular) Antibody (#ARR-022).
4-6. Anti-RAMP2 (extracellular) Antibody, preincubated with RAMP2 (extracellular) Blocking Peptide (#BLP-RR022).
Western blot analysis of rat spleen (lanes 1 and 3) and cortex (lanes 2 and 4):
1,2. Anti-RAMP2 (extracellular) Antibody (#ARR-022), (1:200).
3,4. Anti-RAMP2 (extracellular) Antibody, preincubated with RAMP2 (extracellular) Blocking Peptide (#BLP-RR022).

Indirect flow cytometry

Mouse J774 macrophage cells (5 μg/0.5x10⁶ cells).

Scientific background

RAMP2, also known as receptor activity modifying protein 2, is a single-pass transmembrane protein with extracellular N-terminus and intracellular C-terminal tail. RAMP2 heterodimerizes with several G-protein coupled receptors (GPCRs) and alters their function and life cycle. RAMPs were first identified for the receptors association with calcitonin receptor-like receptor (Calcrl, CLR) as the canonical receptor for the endocrine peptide adrenomedullin (Adm, AM). In addition, RAMP2 also binds to other class B GPCRs: calcitonin receptor (Ctr), corticotrophin-releasing hormone receptor 1 (Crhr1), glucagon receptor (Gcgr), parathyroid hormone receptor 1 (Pth1r), and vasoactive intestinal polypeptide receptor types 1 and 2 (VPAC1 and VPAC2). RAMPs have an important role in receptor trafficking, including translocation from the endoplasmic reticulum to the Golgi, internalization, and recycling of the receptors^1,2.

The mammalian RAMP family includes three members, each encoded by single genes: RAMP1, RAMP2, and RAMP3. Studies have shown that Ramp2 null mice are embryonic lethal, with cardiovascular developmental defects in the heart, blood, and lymphatic vasculatures³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

RAMP2 (extracellular) Blocking Peptide (#BLP-RR022) is the original antigen used for immunization during Anti-RAMP2 (extracellular) Antibody (#ARR-022) generation. The blocking peptide binds and ‘blocks’ Anti-RAMP2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

RAMP2 (extracellular) Blocking Peptide (#BLP-RR022)

Shipping and Ordering Information

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Certificate of Analysis

Anti-RAMP2 (extracellular) Antibody