Overview
- Peptide RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat HRH2 (Accession P25102). 2nd extracellular loop.
- Rat brain and basophilic leukemia (RBL) lysates (1:200).
Western blot analysis of rat basophilic leukemia cells (RBL) (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1,2. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002), (1:200).
3,4. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody, preincubated with Histamine H2 Receptor/HRH2 (extracellular) Blocking Peptide (#BLP-HR002).
- Rat stomach sections (paraffin) (1:100) and mouse brain sections (frozen).
- Mouse primary microglia (Apolloni, S. et al. (2017) Front. Immunol. 8, 1689.).
- Live intact rat basophilic leukemia cell line (1:20).
- The control antigen is not suitable for this application.
Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1,2
The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
H2 receptors couple to adenylate cyclase via Gs, leading to the formation of cAMP and the activation of protein kinase A (PKA).1,2 H2 receptors are expressed in several tissues including the brain, gastrointestinal tract and heart.
The most studied physiological role of the H2 receptor is its involvement in the regulation of gastric acid secretion.3 Specific H2 receptor antagonists are currently used for the treatment of acid-related gastrointestinal conditions such as peptic ulcer, gastroesophageal reflux disease and dyspepsia.
Involvement of H2 receptors in chronic heart failure and immune regulation has also been suggested.
Expression of Histamine H2 Receptor in mouse primary microglia.Immunocytochemical staining of mouse primary microglia using Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002). H2R expression (green) partially co-localizes with CD11b (red).Adapted from Apolloni, S. et al. (2017) Front. Immunol. 8, 1689. with permission of Frontiers.
