Name: Anti-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody
Brand: Alomone Labs
SKU: ACC-029-AG

Overview

Cat #: ACC-029-AG

Alternative Name Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C₆₁₆) with serine (*S) (Accession O35433). 3^rd extracellular loop.

Accession (Uniprot) Number O35433

Gene ID 83810

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat - 14 out of 15 amino acid residues identical. Mouse - 12 out of 15 amino acid residues identical.
Won't recognize TRPV1 from human samples.

RRID AB_2040259.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Specificity Recognizes TRPV1 from rat only.

Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-029), and immunohistochemistry (labeled antibody).

Applications: fc, ic, if, ih, lci

Immunohistochemistry

Rat DRG frozen section (1:60).
Multiplex staining of TRPV1 and Na_V1.8 in rat DRG
Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG), (green), (1:60) and Anti-Na_V1.8 (SCN10A)-ATTO Fluor-594 Antibody (#ASC-016-AR), (red), (1:60). A. TRPV1 staining. B. Na_V1.8 staining. C. Merge of A and B demonstrates partial co-localization of TRPV1 and Na_V1.8 channels. Nuclei stained using DAPI as the counterstain (blue).

Direct flow cytometry

Cell surface detection of TRPV1 by direct flow cytometry in live intact mouse BV-2 microglia cell line:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-ATTO 488 (#RIC-001-AG).
^___ Cells + Anti-TRPV1 (VR1) (extracellular )-ATTO Fluor-488 Antibody (#ACC-029-AG) (2.5μg).

Live cell imaging / Immunocytochemistry

Rat PC12 cells (1:25).

Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.¹

According to IUPHAR the TRP family comprises three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).^1-4 The TRPV subfamily consists of six members, TRPV1-6.⁵

TRPV1 channel has many activators; among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.^6,7TRPV1 is expressed predominantly in nociceptors and in sensory neurons.

Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.^8,9

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Anti-Rat TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody Multiplex staining of TRPV1 and mGluR5 in rat DRGImmunohistochemical staining of rat dorsal root ganglion using Anti-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG), (1:60) and Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (1:60). A. TRPV1 staining (green). B. mGluR5 staining of the same section (red). C. Merge of A and B demonstrates co-localization of TRPV1 and mGluR5 in DRG cells. Nuclei were stained using DAPI as the counterstain (blue).

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Certificate of Analysis

Anti-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

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