Name: Anti-TRPV1 (VR1) (extracellular) Antibody
Brand: Alomone Labs
SKU: ACC-029

Overview

Cat #: ACC-029

Alternative Name Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)NSLPMESTPHK(S)RGS, corresponding to amino acid residues 605-619 of rat TRPV1 (Accession O35433). 3^rd extracellular loop.

Accession (Uniprot) Number O35433

Gene ID 83810

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat - 14 out of 15 amino acid residues identical. Mouse - 12 out of 15 amino acid residues identical.
Won't recognize TRPV1 from human samples.

RRID AB_2040258.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ifc, ih, lci, wb

May also work in: ip*

Western blot

Rat dorsal root ganglion (DRG) lysate (1:200).
Western blot analysis of TRPV1 in rat DRG:
1. Anti-TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:200).
2. Anti-TRPV1 (VR1) (extracellular) Antibody, preincubated with TRPV1/VR1 (extracellular) Blocking Peptide (#BLP-CC029).

Immunohistochemistry

Rat dorsal root ganglion (DRG) frozen section (1:100).

Indirect flow cytometry

Cell surface detection of TRPV1 by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (2.5μg) + goat-anti-rabbit-FITC.

Live cell imaging / Immunocytochemistry

F11, rat DRG cell line (1:1000) (Goswami, C. et al. (2010) J. Cell Sci. 123, 2045.).

Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.¹

According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).^1-4 The TRPV subfamily consists of six members, TRPV1-6.⁵

TRPV1 channel has many activators among them heat, protons, vanilloids like capsaicin, reiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.^6,7

TRPV1 is expressed predominantly in nociceptors and in sensory neurons.

Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.^8,9

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Anti-Rat TRPV1 (VR1) (extracellular) Antibody
Expression of TRPV1 in rat TGNs.Immunocytochemical staining of living rat trigeminal ganglia neurons (TGNs). Extracellular staining of cells using Anti-TRPV1 (VR1) (extracellular) Antibody (#ACC-029). Adapted from Meng, J. et al. (2016) Sci. Rep. 6, 21226. with permission of Nature Publishing Group.

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Anti-TRPV1 (VR1) (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

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