Overview
- Peptide (C)RHKHLAKDHSPDLYS, corresponding to amino acid residues 200 - 214 of mouse Renin Receptor (Accession Q9CYN9). Extracellular, N-terminus.
Western blot analysis of rat heart membranes (lanes 1 and 4), mouse kidney membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):1-3. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200).
4-6. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody, preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4):1, 2. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200).
3, 4. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody, preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
Expression of Renin Receptor in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Renin Receptor immunoreactivity (green) appears in neuronal profiles in the purkinje cell layer (arrows). B. Pre-incubation of the antibody with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (BLP-AR014), suppressed staining. Cell nuclei are stained with DAPI (blue).
Cell surface detection of Renin Receptor in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (2.5 µg) + goat-anti-rabbit-FITC.
- Ichihara, A. and Yatabe, M.S. (2019) Nat. Rev. Nephrol. 15, 693.
The (pro) Renin receptor, (p)RR, encoded by ATP6AP2 gene in humans, is a single pass transmembrane protein that binds both Renin and its inactive precursor pro-renin. ATP6AP2 is highly conserved among species, and (Pro) Renin Receptor orthologues are expressed in species from mammals to C. elegans and Drospohila melanogaster1.
Binding of Pro-renin to (p)RR receptor facilitates its truncation and generation of Renin. Renin is an aspartyl protease that cleaves angiotensinogen into angiotensin I, the rate-limiting reaction in the cascade generating angiotensin, a peptide hormone that is involved in blood pressure regulation. Renin binding by (P)RR affects important biological systems and pathways that are involved in cell cycle regulation, autophagy, acid-base balance, energy metabolism, embryonic development, T cell homeostasis, water balance, blood pressure regulation, cardiac remodeling and maintenance of podocyte structure1.
In addition, (P)RR has been reported to contribute to the pathogenesis of diseases such as fibrosis, hypertension, pre-eclampsia, diabetic microangiopathy, acute kidney injury, cardiovascular disease, cancer and obesity1.
The receptor is widely distributed in organs throughout the body, including the kidneys, heart, brain, eyes, placenta, and the immune system1.
Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize ATP6AP2 from rat, mouse, and human samples. This antibody will recognize both the membrane form of the Renin Receptor and the soluble cleaved form (sPRR).
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