Overview
- Peptide (C)ERSDSPEI(S)HYEKSFHK, corresponding to amino acid residues 138 - 154 of mouse RGMA (Accession Q6PCX7). Extracellular, N-terminus (pro-domain).
RGMA (extracellular) Blocking Peptide (BLP-NR126)
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-RGMA (extracellular) Antibody (#ANR-126), (1:500).
3-4. Anti-RGMA (extracellular) Antibody, preincubated with RGMA (extracellular) Blocking Peptide (BLP-NR126).
Western blot analysis of human glioblastoma U-87 MG cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4):1-2. Anti-RGMA (extracellular) Antibody (#ANR-126), (1:500).
3-4. Anti-RGMA (extracellular) Antibody, preincubated with RGMA (extracellular) Blocking Peptide (BLP-NR126).
Expression of RGMA in mouse hippocampus:Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-RGMA (extracellular) Antibody (#ANR-126), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed RGMA immunoreactivity (green) in neuronal outlines (arrows) in the hilus. B. Pre-incubation of the antibody with RGMA (extracellular) Blocking Peptide (BLP-NR126), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of RGMA in mouse cerebellum:Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-RGMA (extracellular) Antibody (#ANR-126), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. RGMA immunoreactivity (green) appears in purkinje cells (arrows) and their dendrites in the molecular layer (MOL). B. Pre-incubation of the antibody with RGMA (extracellular) Blocking Peptide (BLP-NR126), suppressed staining. Cell nuclei are stained with DAPI (blue).
Cell surface detection of RGMA by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-PE.
___ Cells + Anti-RGMA (extracellular) Antibody (#ANR-126), (2.5μg) + goat-anti-rabbit-PE
- Korecka, J.A. et al. (2017) J. Neurosci., 37, 9361-9379.
- Babitt, J.L. et al. (2005) J. Biol. Chem., 280, 29820-29827.
- Matsunaga, E. et al. (2006) J. Neurosci., 26, 6082-6088.
- Demicheva, E. et al. (2015) Cell Rep., 10, 1887-1898.
- Tanabe, S. and Yamashita, T. (2014) Cell Rep., 9, 1459-1470.
Repulsive Guidance Molecule A (RGMA) is a member of the RGM family, which also includes RGMb (DRAGON) and RGMc (hemojuvelin). It is a glycosylphosphatidylinositol (GPI)-anchored protein characterized by a von Willebrand factor type D domain and a GPI-anchor, facilitating membrane association and soluble forms¹ ². This dual functional nature enables RGMA to act both as a localized, membrane-bound signal and as a long-range soluble signaling molecule, expanding its versatility in biological processes³.
RGMA plays a critical role in axonal guidance, neuronal differentiation, and survival, primarily by interacting with the receptor neogenin. This interaction also regulates apoptosis via Akt pathway dephosphorylation. RGMA acts as a Bone Morphogenetic Protein (BMP) co-receptor, enhancing Smad-dependent signaling pathways² ³. It is expressed in the central nervous system (CNS), particularly in retinal ganglion cells, midbrain dopaminergic neurons, and the spinal cord, with roles extending from embryonic development to adult neuroplasticity¹ ³ ⁴.
RGMA is implicated in several biological processes, including axonal regeneration and inhibition, neuronal survival, and inflammatory responses. Pathological upregulation of RGMA has been linked to neurodegenerative disorders like multiple sclerosis (MS) and Parkinson’s disease. In MS, RGMA inhibits axonal regeneration, while in Parkinson’s, it contributes to dopaminergic neuron degeneration¹ ⁴ ⁵. Research areas benefiting from RGMA immunodetection include neuroregeneration, CNS repair, and therapeutic strategies for neurodegenerative diseases⁴ ⁵.
Anti-RGMA (extracellular) Antibody (#ANR-126) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize RGMA from mouse, rat and human samples.
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