Name: Anti-RIM1 Antibody
Brand: Alomone Labs
SKU: AIP-014

Overview

Cat #: AIP-014

Alternative Name Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)RAKRGRSAESLHMTSE, corresponding to amino acid residues 1039-1054 of rat RIM1 (Accession Q9JIR4). Intracellular.

Accession (Uniprot) Number Q9JIR4

Gene ID 84556

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical.

RRID AB_2756653.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.85 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
1,2. Anti-RIM1 Antibody (#AIP-014), (1:200).
3,4. Anti-RIM1 Antibody, preincubated with RIM1 Blocking Peptide (#BLP-IP014).

References

Han, Y. et al. (2015) J. Neurophysiol. 113, 255.
Su, S.C. et al. (2012) Neuron 75, 675.
Weiss, N. et al. (2011) Pflugers Arch. 461, 447.

Scientific background

RIM1 is a member of the RIM superfamily of proteins (Rim1-4). These genes express seven known RIM isoforms: RIM1α, 1β; RIM2α, 2β, 2γ; and RIM3γ and RIM4γ which serve as scaffolding proteins in presynaptic nerve terminals by interacting with several other protein components localized at the active zone, including Munc13, ELKS (or CAST), liprins and voltage-gated Ca²⁺ channels (VGCCs). RIM1 directly binds to the C-terminal regions of the α₁ subunit of N- and P/Q-type calcium channels, and can also interact with the β auxiliary subunit of P/Q-type calcium channels^1-3_.

RIM proteins were found to be required for normal Ca²⁺ triggering of exocytosis. This interaction with Ca²⁺ channel significantly slows channel inactivation resulting in increased Ca²⁺ influx during action potentials.

Deficiency of RIM1 decreases presynaptic Ca²⁺ channel density. RIM proteins are involved in neurotransmitter release and contribute to long- and short-term synaptic plasticity.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat RIM1. Anti-RIM1 Antibody (#AIP-014) can be used in western blot analysis. It has been designed to recognize RIM1 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

RIM1 Blocking Peptide (#BLP-IP014) is the original antigen used for immunization during Anti-RIM1 Antibody (#AIP-014) generation. The blocking peptide binds and ‘blocks’ Anti-RIM1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

RIM1 Blocking Peptide (#BLP-IP014)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-RIM1 Antibody