Name: Anti-S1PR5/EDG8 (extracellular) Antibody
Brand: Alomone Labs
SKU: ASR-015

Overview

Cat #: ASR-015

Alternative Name S1P receptor 5, S1P5, Endothelial differentiation G-protein-coupled receptor 8, Lysophospholipid receptor B4, Sphingosine 1-phosphate receptor Edg-8, S1P receptor Edg-8

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KLRGARYQPGAGLRAD, corresponding to amino acid residues 24-39 of mouse S1PR5 (EDG8) (Accession Q91X56). Extracellular, N-terminus.

Accession (Uniprot) Number Q91X56

Gene ID 94226

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat and human - identical.

RRID AB_2876815

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of rat cortex (lanes 1 and 5), rat cerebellum (lanes 2 and 6), rat hippocampus (lanes 3 and 7) and mouse brain (lanes 4 and 8) lysates:
1-4. Anti-S1PR5/EDG8 (extracellular) Antibody (#ASR-015), (1:200).
5-8. Anti-S1PR5/EDG8 (extracellular) Antibody, preincubated with S1PR5/EDG8 (extracellular) Blocking Peptide (#BLP-SR015).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human NK-92 natural killer cell line lysate (lanes 2 and 5) and human HL-60 promyelocytic leukemia cell line lysate (lanes 3 and 6) lysates:
1-3. Anti-S1PR5/EDG8 (extracellular) Antibody (#ASR-015), (1:200).
4-6. Anti-S1PR5/EDG8 (extracellular) Antibody, preincubated with the negative control antigen.

Indirect flow cytometry

Cell surface detection of S1PR5 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Goat-anti-rabbit-FITC.
^___ Cells + Anti-S1PR5/EDG8 (extracellular) Antibody (#ASR-015), (2.5µg) + goat-anti-rabbit-FITC

References

Kihara, Y. et al. (2014) Br. J. Pharmacol. 171, 3575.
Meacci, E. and Garcia-Gil, M. (2019) Int. J. Mol. Sci. 20, 6364.
Wang, P. et al. (2019) Cancer Cell Int. 19, 295.
Stepanovska, B. and Huwiler, A. (2019) Pharmacol. Res.

Scientific background

Sphingosine-1-phosphate receptor 5 also known as S1PR5 (EDG8) is a G protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P).

Lysophospholipids encompass a broad range of small, membrane-derived phospholipids that act as extracellular signals, mediated through specific receptors¹. S1PR5 is a high-affinity cell surface S1P receptor, a member of the S1PR family. Like all GPCRs it has the classical structure of seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal¹.

The signaling pathways triggered by S1P/S1P binding play a critical regulatory role in many pathophysiological processes, including skeletal muscle and nervous system degeneration, inflammation, cancer, and autoimmune disorders and hence this interaction has important implications for clinical applications and for personalized medicine^2,3. Targeting S1P receptors and their downstream signaling cascades is considered as a potential treatment for cancer and various autoimmune diseases⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-S1PR5/EDG8 (extracellular) Antibody (#ASR-015) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize EDG8 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

S1PR5/EDG8 (extracellular) Blocking Peptide (#BLP-SR015) is the original antigen used for immunization during Anti-S1PR5/EDG8 (extracellular) Antibody (#ASR-015) generation. The blocking peptide binds and ‘blocks’ Anti-S1PR5/EDG8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

S1PR5/EDG8 (extracellular) Blocking Peptide (#BLP-SR015)

Shipping and Ordering Information

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Anti-S1PR5/EDG8 (extracellular) Antibody