Name: Anti-SAP-97/DLG1 Antibody
Brand: Alomone Labs
SKU: APZ-010

Overview

Cat #: APZ-010

Alternative Name Synapse-associated protein 97, Discs large homolog 1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EYRSKLSQTEDRQLR, corresponding to amino acid residues 17-31 of human SAP-97 (Accession Q12959). N-terminus, L27 domain.

Accession (Uniprot) Number Q12959

Gene ID 1739

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse - identical.

RRID AB_2756771.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):
1-3. Anti-SAP-97/DLG1 Antibody (#APZ-010), (1:200).
4-6. Anti-SAP-97/DLG1 Antibody, preincubated with SAP-97/DLG1 Blocking Peptide (#BLP-PZ010).

References

Fourie, C. et al. (2014) Biochim. Biophys. Acta 1838, 589.
Leonard, A.S. et al. (1998) J. Biol. Chem. 273, 19518.
Howard, M.A. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 3805.
Sheng, M. and Sala, C. (2001) Annu. Rev. Neurosci. 24, 1.
Kim, E. and Sheng, M. (1996) Neuropharmacology 35, 993.
Gardoni, F. et al. (2007) J. Biol. Chem. 282, 28691.
El Haou, S. et al. (2009) Circ. Res. 104, 758.
Leonoudakis, D. et al. (2004) J. Biol. Chem. 279, 22331.
Leonoudakis, D. et al. (2001) J. Cell Sci. 114, 987.

Scientific background

SAP-97 belongs to the MAGUK (membrane associated guanylate kinase) homologs along with PSD-95, PSD-93 and SAP102.

Like other members, SAP-97 has three PDZ domains, one SH3 domain and an inactive GUK domain. Alternative splicing of SAP-97 gives to a number of different isoforms thereby complicating the understanding of its roles¹.

In the post-synaptic cell, SAP-97 is the only MAGUK to directly interact with the GluA1 AMPA receptor². SAP-97 also directly interacts with NMDA receptors through the PDZ domain^3,4.

SAP-97 was shown to interact with K_V1.4⁵ and with K_V4.2 where it directs the channel to spines in hippocampal neurons⁶. It can also interact with the channel in the heart⁷.

Interactions with K_ir channels have also been observed^8,9.

Thus, SAP-97 serves as a regulator of neuronal excitability and synaptic function. Roles for the protein in schizophrenia, Alzheimer’s and Parkinson’s disease and epilepsy have been found¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human SAP-97. Anti-SAP-97/DLG1 Antibody (#APZ-010) can be used in western blot analysis. It has been designed to recognize SAP-97 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

SAP-97/DLG1 Blocking Peptide (#BLP-PZ010) is the original antigen used for immunization during Anti-SAP-97/DLG1 Antibody (#APZ-010) generation. The blocking peptide binds and ‘blocks’ Anti-SAP-97/DLG1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SAP-97/DLG1 Blocking Peptide (#BLP-PZ010)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-SAP-97/DLG1 Antibody