Overview
- Peptide (C)NSFNERRHAYLLKLK, corresponding to amino acid residues 129 - 143 of rat Secretin Receptor (Accession P23811). Extracellular, N-terminus.
- Rat and mouse brain membranes; rat pancreas lysate; mouse kidney menbranes; human PANC-1 pancreatic carcinoma cell lysate (1:200-1:1000).
Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat pancreas lysate (lanes 3 and 7) and mouse kidney membranes (lanes 4 and 8):1-4. Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200).
5-8. Anti-Secretin Receptor (extracellular) Antibody, preincubated with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026).
- Mouse and rat brain sections (1:200).
Secretin receptor, SCTR, binds Secretin, a hormone that affects water homeostasis throughout the body and specifically in the duodenum of the small intestine.
SCTR is ubiquitously expressed throughout the body. It belongs to class B1 of G-protein coupled receptors, along with vasoactive intestinal polypeptide (VIP) receptor. Like all G-protein coupled receptors, it is composed of seven highly conserved transmembrane domains1. The amino terminus of Secretin receptor plays a key role in agonist recognition and responsiveness2.
The binding of SCTR to Secretin initiates a signaling cascade that stimulates bicarbonate secretion which in turn leads to neutralization of gastric H+. This process plays an essential role in fat digestion by creating a more neutral (pH 6 to 8) environment. Secretin levels are regulated by H+ and fatty acids levels in the duodenum and hence the secretion regulation is tightly bound to its activity3.
In addition, SCTR-Secretin interaction plays a major role in the regulation of the secretory activity in the heart, liver and the central nervous system (CNS)3.
