Name: Anti-SERCA3 Antibody
Brand: Alomone Labs
SKU: ACP-014

Overview

Cat #: ACP-014

Alternative Name Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, Calcium pump 3, ATP2A3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)DEALKYLSRHHVD, corresponding to amino acid residues 981-993 of rat SERCA3 (Accession P18596). Cytoplasmic, C-terminus.

Accession (Uniprot) Number P18596

Gene ID 25391

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 11/13 amino acid residues identical; human – 10/13 amino acid residues identical.

RRID AB_2756568.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
1,2. Anti-SERCA3 Antibody (#ACP-014), (1:200).
3,4. Anti-SERCA3 Antibody, preincubated with SERCA3 Blocking Peptide (#BLP-CP014).

Immunohistochemistry

Expression of SERCA3 in rat cerebellum
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SERCA3 Antibody (#ACP-014), (1:200), followed by goat anti-rabbit-AlexaFluor-488. SERCA3 immunoreactivity (green) appears in the molecular layer (MOL) and Purkinje cells (arrows) but not in the granule layer (GL) or cerebellum sections. Cell nuclei are stained with DAPI (blue).

References

Elaïb, Z. et al. (2016) Blood 128, 1129.
Lipskaia, L. et al. (2014) Biochim. Biophys. Acta 1843, 2705.
Erkasap, N. (2007) Anat. J. Cardiol. 1, 43.

Scientific background

SERCA3 belongs to the family of P-type ion translocating ATPases and is responsible for maintaining a Ca²⁺ concentration gradient between the cytosol and the endoplasmic reticulum. SERCA3 transports two Ca²⁺ ions in the endoplasmic reticulum (ER) per ATP molecule hydrolyzed^1-3. SERCA proteins are transmembrane and consist of a single polypeptide chain folded into four major domains. SERCA proteins are encoded by 3 genes, ATP2A1, ATP2A2, and ATP2A3. All three genes produce several alternate transcripts and protein isoforms.

SERCA3 protein is mostly expressed in non-muscle cells, especially in endothelial and hematopoietic cells. Minor expression can be detected in muscle cells²^,3.

SERCA3 knockout mice exhibit prolonged tail bleeding time and rebleeding¹. Mutations in the SERCA3 gene are associated with type 2 diabetes. The protein is also implicated in several cardiac disorders^2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-SERCA3 Antibody (#ACP-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SERCA3 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

SERCA3 Blocking Peptide (#BLP-CP014) is the original antigen used for immunization during Anti-SERCA3 Antibody (#ACP-014) generation. The blocking peptide binds and ‘blocks’ Anti-SERCA3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SERCA3 Blocking Peptide (#BLP-CP014)

Shipping and Ordering Information

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Anti-SERCA3 Antibody