Name: Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: ANT-201-F

Overview

Cat #: ANT-201-F

Alternative Name Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CANSSLHDYAKIFP(R), corresponding to amino acid residues 322-335 of human SLC11A1 (Accession P49279). 4^th extracellular loop.

Accession (Uniprot) Number P49279

Gene ID 6556

Homology Human, rat – 14/15 amino acid residues identical; mouse – 13/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANT-201), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of SLC11A1/NRAMP1 by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody (#ANT-201-F), (2.5µg).
Cell surface detection of SLC11A1/NRAMP1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody (#ANT-201-F), (5µg).

References

Blackwell, J.M. et al. (2003) Immunol. Lett. 85, 197.
Zwilling, B.S. et al. (1999) Infect. Immunol. 67, 1386.
Goswami, T. et al. (2001) Biochem. J. 354, 511.
Qi, H. et al. (2014) Spectrochim. Acta A Mol. Biomol. Spectrosc. 122, 82.

Scientific background

Solute carrier family 11 member a1 (Slc11a1) also known as natural resistance-associated macrophage protein 1 (NRAMP1) is a proton/divalent cation antiporter, known for its role in regulating the antimicrobial activity of macrophages¹. The structure of Slc11a1 includes a common and conserved hydrophobic core of 10 transmembrane domains (TM) and either one or two non-conserved, highly hydrophobic TM domains⁴.

Slc11a1 delivers divalent cations such as Fe²⁺, Zn²⁺, and Mn²⁺ from the cytosol to the late endosome/lysosome acidic compartments where the Fenton reaction can use ferrous iron to generate toxic hydroxyl radicals². Thus, providing one mechanism by which Slc11a1 could influence antimicrobial activity in macrophages.

Transition of metal ions is essential and participates in numerous cellular functions such as regulation of transcription. In addition, hundreds of enzymes require metal ions as co-factors³.

Studies show that polymorphism in SLC11A1 is correlated to the occurrence of autoimmune and infectious disease susceptibility, as well as innate and adaptive immune responses to mycobacterial products/antigens. Therefore, research on this ion transporter is of high importance¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize NRAMP1 from rat, mouse, and human samples.

Anti- SLC11A1/NRAMP1 (extracellular)-FITC Antibody (#ANT-201-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

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Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody