Overview
- Peptide (C)KGEVQSWALSDHHG, corresponding to amino acid residues 479-492 of mouse SLC17A5 (Accession Q8BN82). Intracellular, C-terminus.
Western blot analysis of rat small intestine (lanes 1 and 3) and brain (lanes 2 and 4) lysates:1,2. Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200).
3,4. Anti-SLC17A5 (Sialin) Antibody, preincubated with SLC17A5/Sialin Blocking Peptide (#BLP-ST015).
Western blot analysis of mouse colon lysate:1. Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200).
2. Anti-SLC17A5 (Sialin) Antibody, preincubated with SLC17A5/Sialin Blocking Peptide (#BLP-ST015).
Western blot analysis of human HT-29 colorectal adenocarcinoma (lanes 1 and 3) and human HepG2 liver hepatome (lanes 2 and 4) cell lysates:1,2. Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200).
3,4. Anti-SLC17A5 (Sialin) Antibody, preincubated with SLC17A5/Sialin Blocking Peptide (#BLP-ST015).
Expression of Sialin in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. SLC17A5 immunoreactivity (green) appears in the neuropil of the ventral horn. Cell nuclei are stained with DAPI (blue).
- Tarailo-Graovac, M. et al. (2017) Orphanet. J. Rare Dis. 12, 28.
- Lines, M.A. et al. (2014) JIMD Rep. 12, 79.
- Courville, P. et al. (2010) J. Biol. Chem. 285, 19316.
Sialin, encoded by the SLC17A5 gene, is a member of the SLC17 solute carrier family, a group of structurally related polytopic membrane proteins, part of the major facilitator superfamily of transporters. Sialin is a sialic acid transporter located in the lysosomal membrane. In the lysosome, sialic acid residues are sequentially removed from the carbohydrate chain by sialidases and then transported out of the lysosome by the transporter protein sialin1,2. Sialin structure consists of 12 transmembrane helices (TMs). TM4 contains three residues (Arg-168, Glu-175, and His-183) that are highly conserved in the SLC17 family, suggesting that they may participate directly in substrate binding and translocation. In addition, TM4 contains a GXXXG helix packing motif that is important for the expression of sialin. SLC17 family includes several more GXXXG-like motifs located within TMs including GXXXG/S in TM2, G/AXXXG in TM5, and G/S/AXXXGXXXG/T in TM13.
Proteins included in the SLC17 family are implicated with inherited neurological and metabolic diseases. Mutations in the SLC17A5 gene cause sialic acid storage diseases including infantile sialic acid storage disease and Salla disease. These conditions are autosomal recessive neurodegenerative disorders characterized by excessive accumulation of sialic acid in the lysosome1,2.
Anti-SLC17A5 (Sialin) Antibody (#AST-015) is a highly specific antibody directed against an epitope of the mouse Sialin transporter. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Sialin from rat, mouse, and human samples.
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