Name: Anti-SLC19A1/RFC1 (extracellular) Antibody
Brand: Alomone Labs
SKU: AFT-001

Overview

Cat #: AFT-001

Alternative Name Solute Carrier Family 19 Member 1, Reduced Folate Carrier 1, Reduced Folate Transporter 1, RFT1, Intestinal folate carrier 1, IFC-1, Reduced Folate Transporter, CHMD, FOLT, REFC, RFC, RFT-1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)SDKRGLGLQVRDQFR, corresponding to amino acid residues 412 - 426 of mouse SLC19A1 (Accession P41438). 6^th extracellular loop.

Accession (Uniprot) Number P41438

Gene ID 20509

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – 13/15 amino acid residues identical; human – 11/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Applications: ifc, ih, wb

May also work in: ic*, ip*, lci*

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat small intestine lysate (lanes 3 and 7) and rat liver membranes (lanes 4 and 8):
1-4. Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (1:400).
5-8. Anti-SLC19A1/RFC1 (extracellular) Antibody, preincubated with SLC19A1/RFC1 (extracellular) Blocking Peptide (#BLP-FT001).
Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human K562 myelogenous leukemia cell line lysate (lanes 2 and 4):
1, 2. Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (1:400).
3, 4. Anti-SLC19A1/RFC1 (extracellular) Antibody, preincubated with SLC19A1/RFC1 (extracellular) Blocking Peptide (#BLP-FT001).

Immunohistochemistry

Expression of SLC19A1 in rat hippocampus.
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA3 region, showed immunoreactivity (green) in neuronal profiles in the pyramidal layer (arrows). B. Pre-incubation of the antibody with SLC19A1/RFC1 (extracellular) Blocking Peptide (BLP-FT001), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.

Indirect flow cytometry

Cell surface detection of SLC19A1 in live intact human Jurkat T-cell leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (5 µg) + goat-anti-rabbit-FITC.

References

Prasad, P.D. et al. (1995) Biochem. Biophys. Res. Commun. 206, 681.
Yee, S.W. et al. (2011) Pharmacogenet. Genomics 20, 708.
Cao, W. and Matherly, L.H. (2004) Biochem. J. 378, 201.
Ifergan, I. et al. (2008) J. Biol. Chem. 283, 20687.
Whetstine, J.R. et al. (2002) Biochem. J. 367, 629.
Hu, Y.H. et al. (2019) Curr. Pharm. Des. 25, 627.

Scientific background

Solute Carrier Transporter 19A1, (SLC19A1, RFC1) is a high-capacity, bi-directional transporter of 5-methyl-tetrahydrofolate and thiamine monophosphate^1,2. SLC19A1 is characterized by 12 transmembrane domains (TMDs) and cytoplasm oriented N- and C-termini³.

Folate is a major nutrient that supports important physiological functions such as DNA synthesis, cell division and substrate methylation. Low folate levels caused by suboptimal intake, transport and cellular utilization of folate, is a risk factor for diseases such as spina bifida, cardiovascular diseases and certain types of cancer¹.

SLC19A1 is the main folate transporter and it plays a critical role in folate uptake and homeostasis in mammalian cells⁴. High SLC19A1 transcript levels are detected in liver and placenta, with appreciable levels in other tissues, including kidney, lung, bone marrow, intestine, brain, and portions of the central nervous system⁵.

SLC19A1 is also involved in the uptake of Methotrexate (MTX); one of the leading chemotherapeutic agents with the best demonstrated efficacies against childhood acute lymphoblastic leukemia and hence malfunction of this transporter also effects the efficiency of this type of cancer treatment⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SLC19A1 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

SLC19A1/RFC1 (extracellular) Blocking Peptide (#BLP-FT001) is the original antigen used for immunization during Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001) generation. The blocking peptide binds and ‘blocks’ Anti-SLC19A1/RFC1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SLC19A1/RFC1 (extracellular) Blocking Peptide (#BLP-FT001)

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Anti-SLC19A1/RFC1 (extracellular) Antibody