Name: Anti-SLC28A3/CNT3 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANT-063

Overview

Cat #: ANT-063

Alternative Name Solute Carrier Family 28 Member 3, Concentrative Na+-Nucleoside Cotransporter 3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KITLKNAMKMENGDSR, corresponding to amino acid residues 432 - 447 of rat SLC28A3/CNT3 (Accession Q8VIH3). 5^th extracellular loop.

Accession (Uniprot) Number Q8VIH3

Gene ID 140944

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 15/16 amino acid residues identical; human – 14/16 amino acid residues identical.

RRID AB_2876837

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, wb

May also work in: ic*, ih*, ip*, lci*

Western blot

Mouse kidney and brain membrane lysates; rat pancreas and brain lysates; human HL-60 myelocytic leukemia, K562 chronic myelogenous leukemia, MCF-7 breast adenocarcinoma, and PANC-1 pancreatic carcinoma cell lysates (1:400-1:2500).
Western blot analysis of mouse kidney membranes (lanes 1 and 5), rat pancreas lysates (lanes 2 and 6), mouse brain membranes (lanes 3 and 7) and rat brain lysate (lanes 4 and 8):
1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:400).
5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) (#BLP-NT063).
Western blot analysis of human HL-60 myelocytic leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 7) and human PANC-1 pancreatic carcinoma cell line lysate (lanes 4 and 8):
1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:500).
5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) (#BLP-NT063).

Indirect flow cytometry

Cell surface detection of SLC28A3/CNT3 in live intact human HL-60 promyelocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (5 µg) + goat-anti-rabbit-FITC.

References

Badagnani, I. et al. (2005) Pharmacogenomics J. 5, 157.
Stecula, A. et al. (2017) Biochemistry 56, 3475.
Damaraju, S. et al. (2005) Pharmacogenet. Genomics 15, 173.
Gray, J.H. et al. (2004) Pflugers Arch. 447, 728.

Scientific background

Solute carrier family 28 member 3 (SLC28A3, CNT3) is a sodium-dependent, concentrative pyrimidine- and purine-selective transporter. SLC28A3 plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs, in a process that is coupled to the sodium cellular gradient¹.

CNT3 consists of 13 putative trans-membrane domains and it forms a homo-trimer which is important for its activity as a transporter^2,3.

SLC28 members are crucial determinants in the response to a variety of anticancer and antiviral nucleoside analogs, as they modulate the entry of these analogs into target tissues. Within this large group of solute carriers, SLC28A is a sub-group which consists of SLC28A1, SLC28A2, and SLC28A3. Of the three members of this sub-family, SLC28A3 has the broadest selectivity and the widest expression profile⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize CNT3 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) is the original antigen used for immunization during Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063) generation. The blocking peptide binds and ‘blocks’ Anti-SLC28A3/CNT3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063)

Shipping and Ordering Information

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Anti-SLC28A3/CNT3 (extracellular) Antibody