Overview
- Peptide (C)KITLKNAMKMENGDSR, corresponding to amino acid residues 432 - 447 of rat SLC28A3/CNT3 (Accession Q8VIH3). 5th extracellular loop.
- Mouse kidney and brain membrane lysates; rat pancreas and brain lysates; human HL-60 myelocytic leukemia, K562 chronic myelogenous leukemia, MCF-7 breast adenocarcinoma, and PANC-1 pancreatic carcinoma cell lysates (1:400-1:2500).
Western blot analysis of mouse kidney membranes (lanes 1 and 5), rat pancreas lysates (lanes 2 and 6), mouse brain membranes (lanes 3 and 7) and rat brain lysate (lanes 4 and 8):1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:400).
5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) (#BLP-NT063).
Western blot analysis of human HL-60 myelocytic leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 7) and human PANC-1 pancreatic carcinoma cell line lysate (lanes 4 and 8):1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:500).
5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) (#BLP-NT063).
Cell surface detection of SLC28A3/CNT3 in live intact human HL-60 promyelocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (5 µg) + goat-anti-rabbit-FITC.
- Badagnani, I. et al. (2005) Pharmacogenomics J. 5, 157.
- Stecula, A. et al. (2017) Biochemistry 56, 3475.
- Damaraju, S. et al. (2005) Pharmacogenet. Genomics 15, 173.
- Gray, J.H. et al. (2004) Pflugers Arch. 447, 728.
Solute carrier family 28 member 3 (SLC28A3, CNT3) is a sodium-dependent, concentrative pyrimidine- and purine-selective transporter. SLC28A3 plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs, in a process that is coupled to the sodium cellular gradient1.
CNT3 consists of 13 putative trans-membrane domains and it forms a homo-trimer which is important for its activity as a transporter2,3.
SLC28 members are crucial determinants in the response to a variety of anticancer and antiviral nucleoside analogs, as they modulate the entry of these analogs into target tissues. Within this large group of solute carriers, SLC28A is a sub-group which consists of SLC28A1, SLC28A2, and SLC28A3. Of the three members of this sub-family, SLC28A3 has the broadest selectivity and the widest expression profile4.
Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize CNT3 from human, rat, and mouse samples.
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