Name: Anti-SLC28A3/CNT3 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANT-063

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Overview

Cat #: ANT-063

Alternative Name Solute Carrier Family 28 Member 3, Concentrative Na+-Nucleoside Cotransporter 3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KITLKNAMKMENGDSR, corresponding to amino acid residues 432 - 447 of rat SLC28A3/CNT3 (Accession Q8VIH3). 5^th extracellular loop.

Accession (Uniprot) Number Q8VIH3

Gene ID 140944

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 15/16 amino acid residues identical; human – 14/16 amino acid residues identical.

RRID AB_2876837

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, wb

May also work in: ic*, ih*, ip*, lci*

Western blot

Mouse kidney and brain membrane lysates; rat pancreas and brain lysates; human HL-60 myelocytic leukemia, K562 chronic myelogenous leukemia, MCF-7 breast adenocarcinoma, and PANC-1 pancreatic carcinoma cell lysates (1:400-1:2500).
Western blot analysis of mouse kidney membranes (lanes 1 and 5), rat pancreas lysates (lanes 2 and 6), mouse brain membranes (lanes 3 and 7) and rat brain lysate (lanes 4 and 8):
1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:400).
5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) (#BLP-NT063).
Western blot analysis of human HL-60 myelocytic leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 7) and human PANC-1 pancreatic carcinoma cell line lysate (lanes 4 and 8):
1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:500).
5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) (#BLP-NT063).

Indirect flow cytometry

Human HL-60 promyelocytic leukemia cells (5 µg).
Cell surface detection of SLC28A3/CNT3 in live intact human HL-60 promyelocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (5 µg) + goat-anti-rabbit-FITC.

Scientific background

Solute carrier family 28 member 3 (SLC28A3, CNT3) is a sodium-dependent, concentrative pyrimidine- and purine-selective transporter. SLC28A3 plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs, in a process that is coupled to the sodium cellular gradient¹.

CNT3 consists of 13 putative trans-membrane domains and it forms a homo-trimer which is important for its activity as a transporter^2,3.

SLC28 members are crucial determinants in the response to a variety of anticancer and antiviral nucleoside analogs, as they modulate the entry of these analogs into target tissues. Within this large group of solute carriers, SLC28A is a sub-group which consists of SLC28A1, SLC28A2, and SLC28A3. Of the three members of this sub-family, SLC28A3 has the broadest selectivity and the widest expression profile⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) is the original antigen used for immunization during Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063) generation. The blocking peptide binds and ‘blocks’ Anti-SLC28A3/CNT3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063)

Shipping and Ordering Information

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Anti-SLC28A3/CNT3 (extracellular) Antibody