Name: Anti-SLC3A1 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANT-100

Overview

Cat #: ANT-100

Alternative Name Neutral and basic amino acid transport protein rBAT, Solute carrier family 3 (cystine, dibasic and neutral amino acid transporters) member 1, B(0,+)-type amino acid transport protein, NBAT, Amino acid transporter 1, ATR1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)KEQPDLNFRNPAVQE, corresponding to amino acid residues 277-291 of rat SLC3A1 (Accession Q64319). Extracellular, C-Terminus.

Accession (Uniprot) Number Q64319

Gene ID 29484

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical; human – 14/15 amino acid residues identical.

RRID AB_2756721.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*, lci*

Western blot

Western blot analysis of rat (lane 1 and 3) and mouse (lanes 2 and 4) kidney lysates:
1,2. Anti-SLC3A1 (extracellular) Antibody (#ANT-100), (1:400).
3,4. Anti-SLC3A1 (extracellular) Antibody, preincubated with SLC3A1 (extracellular) Blocking Peptide (#BLP-NT100).

References

Jiang, Y. et al. (2017) Theranostics 7, 1036.
Nagamori, S. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, 775.
Markazi, S. et al. (2017) J. Res. Med. Sci. 22, 33.

Scientific background

The heteromeric amino acid transporter (HAT) family is one of the major amino acid transporter families responsible for cellular uptake and epithelial transport. The uptake of cysteine mainly depends on HATs. HATs are localized to the plasma membrane and form heterodimers composed of a 12-membrane spanning light chain (SLC7) and a single membrane spanning heavy chain, SLC3. SLC7 catalyzes transport functions while SLC3 is essential for plasma membrane localization and stabilization of the light chains. The SLC3A1 is mainly expressed in the apical plasma membrane in the epithelial cells of intestinal mucosa and the renal proximal tubules^1,2.

Two heavy chains, SLC3A1 and SLC3A2 are covalently bound to the light chains through a disulfide bridge. SLC3A1 has been known to form a heterodimer only with SLC7A9, a family member of light chain^1,2.

Type 1 Cystinuria is associated with mutations in SLC3A1_, an inherited disease characterized by the formation of cystine calculi in the kidneys, ureters, and bladder³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-SLC3A1 (extracellular) Antibody (#ANT-100) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize SLC3A1 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

SLC3A1 (extracellular) Blocking Peptide (#BLP-NT100) is the original antigen used for immunization during Anti-SLC3A1 (extracellular) Antibody (#ANT-100) generation. The blocking peptide binds and ‘blocks’ Anti-SLC3A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SLC3A1 (extracellular) Blocking Peptide (#BLP-NT100)

Shipping and Ordering Information

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Anti-SLC3A1 (extracellular) Antibody