Name: Anti-SLC3A2 (CD98) (extracellular) Antibody
Brand: Alomone Labs
SKU: ANT-192

Overview

Cat #: ANT-192

Alternative Name Solute Carrier Family 3 Member 2, 4F2 Cell-Surface Antigen Heavy Chain, 4F2HC, CD98 Antigen Heavy Chain, CD98HC

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KGLVLGPIHKNQKDE, corresponding to amino acid residues 150 - 164 of mouse SLC3A2 (Accession P10852). Extracellular, C-terminus.

Accession (Uniprot) Number P10852

Gene ID 17254

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical. Human - 14 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody
SLC3A2 (CD98) (extracellular) Blocking Peptide (#BLP-NT192)

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
1-2. Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:200).
3-4. Anti-SLC3A2 (CD98) (extracellular) Antibody, preincubated with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192).
Western blot analysis of mouse liver membranes (lanes 1 and 3) and mouse kidney lysate (lanes 2 and 4):
1-2. Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:200).
3-4. Anti-SLC3A2 (CD98) (extracellular) Antibody, preincubated with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192).
Western blot analysis of human U-87 MG glioma cell line lysate (lanes 1 and 3) and human HUVEC endothelial cell line lysate (lanes 2 and 4):
1-2. Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:400).
3-4. Anti-SLC3A2 (CD98) (extracellular) Antibody, preincubated with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192).

Immunohistochemistry

Expression of SLC3A2 (CD98) in mouse hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA1 region showed SLC3A2 (CD98) immunoreactivity (green), in pyramidal neurons (vertical arrows) and in interneurons (horizontal arrow). B. Pre-incubation of the antibody with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192), suppressed staining. Cell nuclei are stained with DAPI (blue).

Indirect flow cytometry

Cell surface detection of SLC3A2 (CD98) by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-PE.
^___ Cells + Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (5μg) + goat-anti-rabbit-PE.
Cell surface detection of SLC3A2 (CD98) by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-PE.
^___ Cells + Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (5μg) + goat-anti-rabbit-PE.

References

Kahlhofer, J. et al. (2022) Basic Clin. Pharmacol. Toxicol. doi: 10.1111/bcpt.13821.
Nguyen, N. et al. (2018) Sci. Rep. 8,1.
Nachef, M. et al. (2021) Front. Immunol. 12, 624324.
Nguyen, H.T.T. and Merlin, D. (2012) Cell Mol. Life Sci. 69,18.
Liu, L. et al. (2021) Cell Biochem. Funct. 39,174.

Scientific background

Solute carrier family 3 member 2 (SLC3A2), also known as 4F2 cell-surface antigen heavy chain (4F2HC) and CD98 antigen heavy chain (CD98hc), is a type II membrane protein belonging to the SLC3A/CD98 transporter family that plays a role in various physiological processes including amino acid transport, cell differentiation, migration, and proliferation.^1,2,4

SLC3A2 is a type II glycosylated protein composed of an extracellular domain, a transmembrane domain, and a cytoplasmic domain. SLC3A2 forms obligatory heterodimeric complexes with six members of the L-type amino acid transporters (LATs) SLC7A5-11, and SLC7A13.¹

The heteromeric complex between SLC3A2 and SLC7A5 (also known as LAT1) is one of the best characterized. SLC7A5 is responsible for the transport of large neutral amino acids, including leucine, across the cell membrane. SLC3A2 acts as a chaperone protein for SLC7A5, stabilizing its expression on the cell surface and assisting in its proper functioning. This partnership is essential for amino acid transport, cell growth, and protein synthesis. This complex is particularly important in cancer cells and immune cells, as it facilitates nutrient uptake and influences cell proliferation and immune responses.^1-4

In addition to SLC7A5, SLC3A2 also forms a complex with another SLC7 family member called SLC7A11 (also known as xCT). This complex is commonly referred to as the CD98/xCT or SLC3A2/SLC7A11.^1-4

xCT, is a cystine/glutamate antiporter that plays a crucial role in the cellular exchange of cystine (an amino acid involved in the synthesis of the antioxidant glutathione) for glutamate. This exchange is important for maintaining cellular redox balance and protecting cells from oxidative stress.^1,3,5

When SLC3A2 partners with SLC7A11, it facilitates the function of the xCT antiporter, allowing cells to import cystine while exporting glutamate. This process is essential for antioxidant defence and protecting cells from damage caused by reactive oxygen species (ROS). The SLC3A2/SLC7A11 complex has significant implications in cancer biology and the modulation of immune responses, as it can influence cellular redox status and affect processes related to cell proliferation and immune cell function.^1,3,5

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize SLC3A2 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

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Anti-SLC3A2 (CD98) (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Applications

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Scientific Background

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