Name: Anti-SLC41A2 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANT-146

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Overview

Cat #: ANT-146

Alternative Name Solute carrier family 41 member 2, Solute carrier family 41 (magnesium transporter), Member 2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KRSEPHLDYHEFSE, corresponding to amino acid residues 78-91 of mouse SLC41A2 (Accession Q8BYR8). Extracellular, N-terminus.

Accession (Uniprot) Number Q8BYR8

Gene ID 338365

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat- identical; human – 9/14 amino acid residues identical.

RRID AB_2876810

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Rat and mouse brain lysates; human liver carcinoma HepG2 cell lysates (1:1000-1:5000).
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) membranes:
1, 2. Anti-SLC41A2 (extracellular) Antibody (#ANT-146), (1:2000).
3, 4. Anti-SLC41A2 (extracellular) Antibody, preincubated with SLC41A2 (extracellular) Blocking Peptide (#BLP-NT146).

Scientific background

SLC41A2, Solute carrier family 41 member 2, is a magnesium (Mg^2 +) transporter belonging to the SLC41 transporter family, which includes SLC41A1 and SLC41A3¹.

SLC41 proteins appear to possess the same basic structural features of prokaryotic MgtE transporters: they create a Mg²⁺-selective pore via homodimerization. SLC41 topology suggests the presence of two five TM span domains connected by a TM spanning linker. The N-terminal cytoplasmic domain of SLC41A1 regulates its function^2,3.

SLC41A2 is highly expressed in lymph nodes, stomach, lungs, testis and skin and is moderately expressed in spleen, intestine, heart, breast and kidneys².

SLC41A2 is reported as either over or under expressed in 75 disease states including a number of carcinomas and lymphomas according to the database on European Bioinformatics Institute (EBI).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

SLC41A2 (extracellular) Blocking Peptide (#BLP-NT146) is the original antigen used for immunization during Anti-SLC41A2 (extracellular) Antibody (#ANT-146) generation. The blocking peptide binds and ‘blocks’ Anti-SLC41A2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SLC41A2 (extracellular) Blocking Peptide (#BLP-NT146)

Shipping and Ordering Information

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Certificate of Analysis

Anti-SLC41A2 (extracellular) Antibody