Overview
- Peptide (C)RDTYLQFTDLQNWPR, corresponding to amino acid residues 1170 - 1184 of mouse SLIT1 (Accession Q80TR4). Secreted.
SLIT1 Blocking Peptide (#BLP-NR221)
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-SLIT1 Antibody (#ANR-221), (1:200).
3-4. Anti-SLIT1 Antibody (#ANR-221), preincubated with SLIT1 Blocking Peptide (#BLP-NR221).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human Jurkat T cell leukemia cell line lysate (lanes 3 and 6):1-3. Anti-SLIT1 Antibody (#ANR-221), (1:200).
4-6. Anti-SLIT1 Antibody (#ANR-221), preincubated with SLIT1 Blocking Peptide (#BLP-NR221).
Expression of SLIT1 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-SLIT1 Antibody (#ANR-221), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SLIT1 immunoreactivity (green), appears in Purkinje cells (vertical arrows) and their dendritic tree (horizontal arrows). B. Pre-incubation of the antibody with SLIT1 Blocking Peptide (#BLP-NR221), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Blockus, H. and Chédotal, A. (2016) Development. 143, 3037.
- Tong, M. et al. (2019) J. Cancer 10, 2694.
- Gonda, Y. et al. (2020) Front. Cell Dev. Biol. 8, 607415.
- Deboux, C. et al. (2020) Front. Cell. Neurosci. 14, 168.
- Jiang, Z. et al. (2019) Ther. Adv. Med. Oncol. 11, 1758835919855238.
- Koohini, Z. et al. (2019) Pathol. Oncol. Res. 25, 1285.
Slit homolog 1 protein (SLIT1), also known as multiple EGF-like domains protein 4, and MEGF4, is a secreted protein member of the SLIT family involved in axon guidance and cell migration.
SLIT1 is a member of the SLIT family proteins that includes three members: SLIT1, SLIT2 and SLIT3. SLITS are secreted glycoproteins comprised of four stretches of leucine-rich repeat (LRR) domains, nine epidermal growth factor (EGF) repeats, a Laminin-G-like domain, and a C-terminal cysteine knot.1,2
SLITS exert their many physiological roles by binding to Roundabout (Robo) receptors family, that includes four members (Robo 1 to Robo 4), which are single transmembrane receptors belonging to the immunoglobulin superfamily.1,2
SLITS play a crucial role in axon guidance during the development of the nervous system. Axon guidance is the process by which neurons extend their axons to reach their appropriate targets, such as other neurons, muscles, or glands. This process is essential for the formation of functional neural circuits.1,3
In addition, SLIT1 is involved in various aspects of the regulation of neural progenitor cells (NPCs). NPCs are the precursors to neurons and glial cells in the nervous system. They undergo proliferation, migration, and differentiation to generate the diverse cell types found in the mature brain. SLIT1 is involved in several aspects of NPCs functions, including promoting the proliferation of NPCs in several brain regions, guiding NPCs from the sub-ventricular zone towards the cortical plate and supporting the differentiation of NPCs into specific neuronal or glial cell types.3,4
Finally, a complex role for SLIT1 in cancer has emerged. SLIT1 has been ascribed a tumor suppressive role in certain cancers such as breast and colorectal, while in others it appears to associate with tumor progression. In particular, in glioma and pancreatic cancer, SLIT1 expression has been correlated with enhanced metastasis formation and poor prognosis. 2,5,6
Anti-SLIT1 Antibody (#ANR-221) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLIT1 from rat, mouse and human samples.
Powered by Bioz
