Overview
- Peptide (C)RHDEKRNIYQKIRDHDLLD, corresponding to amino acid residues 14-32 of human KCNMB2 (Accession Q9Y691). Intracellular, N-terminal part.
Western blot analysis of rat kidney (lanes 1 and 3) and heart (lanes 2 and 4) membranes:1,2. Anti-sloβ2 (KCNMB2) Antibody (#APC-034), (1:200).
3,4. Anti-sloβ2 (KCNMB2) Antibody, preincubated with sloβ2/KCNMB2 Blocking Peptide (#BLP-PC034).
Expression of KCNMB2 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-sloβ2 (KCNMB2) Antibody (#APC-034). A. KCNMB2 appears in the pyramidal layer (green). B. Staining of interneurons with mouse anti-parvalbumin (PV, red). C. Confocal merge of KCNMB2 and PV demonstrates presence of KCNMB2 also in PV (GABA containing) cells (orange).
- Rat pinealocytes (Mizutani, H. et al. (2016) Am. J. Physiol. 310, C740.)
- Wallner, M. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 4137.
- Xia, X.M. et al. (1999) J. Neurosci. 19, 5255.
- Orio, P. et al. (2002) News Physiol. Sci. 17, 156.
sloβ2 is a member the regulatory β subunit family that controls the activity of the large conductance Ca2+-activated K+ channel KCa1.1. This family includes four members with a shared topology: two trans-membrane domains, short intracellular N- and C-termini and a large extracellular region and a distinct tissue distribution.
sloβ2 expression is relatively broad and includes expression in the brain, heart, kidney, adrenal chromaffin cells and ovary.
The KCa1.1 K+ channel can be activated by either an increase in intracellular Ca2+ concentration or by membrane depolarization. The regulatory β subunits increase the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and membrane voltage and they may also change the channel pharmacology.
The sloβ2 subunit is unique in that it is able to induce a rapid and complete inactivation of the KCa1.1 channel in a manner that closely resembles the ball-and-chain inactivation of the voltage-dependent K+ (KV) channels. In other words, the inactivation is dependent on a sequence in the N-terminal part of the sloβ2 subunit that appears to block the mouth of the ion permeation pathway.
The physiological significance of the sloβ2 subunit is not clear, but it appears to participate in the inactivation of the KCa1.1 channel in hippocampal CA1 neurons and adrenal chromaffin cells.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human sloβ2. Anti-sloβ2 (KCNMB2) Antibody (#APC-034) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize sloβ2 from human, rat, and mouse samples.
Applications
Citations
Powered by Bioz- Human dermal fibroblasts (1:200).
Kicinska, A. et al. (2016) Biochem. J. 473, 4457. - Mitochondria and mitoplast from human endothelial EA.hy926 cells (1:200).
Bednarczyk, P. et al. (2013) Am. J. Physiol. 304, H1415.
- Rat pinealocytes.
Mizutani, H. et al. (2016) Am. J. Physiol. 310, C740.
- Wulf-Johansson, H. et al. (2010) Neuroscience 167, 1091.
- Piwonska, M. et al. (2008) Neuroscience 153, 446.
