Name: Anti-SMCT1 (SLC5A8) Antibody
Brand: Alomone Labs
SKU: AGT-036

Overview

Cat #: AGT-036

Alternative Name Sodium-Coupled Monocarboxylate Transporter 1, Solute Carrier Family 5 Member 8, Apical Iodide Transporter, AIT

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)GGRKQNLDPRYILTK, corresponding to amino acid residues 544 - 558 of human SMCT1 (Accession Q8N695). Intracellular, C-terminus.

Accession (Uniprot) Number Q8N695

Gene ID SLC5A8

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat – 13 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody.

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ip*

Western blot

Western blot analysis (1:200-1:600).
Western blot analysis of mouse brain lysate (lanes 1 and 4), rat kidney membranes (lanes 2 and 5) and mouse colon membranes (3 and 6):
1-3. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 4-6. Anti-SMCT1 (SLC5A8) Antibody, preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).
Western blot analysis of human LoVo colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigmented epithelium cell line lysate (2 and 4):
1-2. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 3-4. Anti-SMCT1 (SLC5A8) Antibody, preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).

Immunohistochemistry

Expression of SMCT1 in rat supraoptic nucleus.
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SMCT1 immunoreactivity (green) was detected in neuronal profiles (arrows). B. Pre-incubation of the antibody with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036), suppressed staining. Cell nuclei are stained with DAPI (blue).

Scientific background

Sodium-coupled monocarboxylate transporter 1, SLC5A8, is an electrogenic sodium (Na⁺) and chloride (Cl-)-dependent sodium-coupled solute transporter that belongs to the Na⁺/glucose co-transporter gene family (SLC5) ¹.

It is expressed in the colon, ileum, kidney and thyroid gland and it is involved in the transport of a variety of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate) ¹.

SLC5A8 was found to be silenced in colon cancer, gastric cancer and possibly other cancer types and hence it is a candidate tumor suppressor gene. This is probably mediated through its role in transforming butyrate, a metabolite that is related to these types of cancer ².

The structure of SLC5A8 was predicted based on its homology to sodium-iodide symporter (NIS) and on the fact that it is a part of the Solut Sodium Symporter (SSS) protein family. The structure contains 13 α-helical TM segments, with the N-terminus facing the extracellular medium and the C-terminus facing the cytoplasm, and a conserved sodium binding site ³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

SMCT1 (SLC5A8) Blocking Peptide (#BLP-GT036) is the original antigen used for immunization during Anti-SMCT1 (SLC5A8) Antibody (#AGT-036) generation. The blocking peptide binds and ‘blocks’ Anti-SMCT1 (SLC5A8) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SMCT1 (SLC5A8) Blocking Peptide (#BLP-GT036)

Shipping and Ordering Information

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Anti-SMCT1 (SLC5A8) Antibody