Overview
- Peptide (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat NALCN (Accession Q6Q760). Extracellular, S1-S2 domain III.
Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and human SH-SYS5 brain neuroblastoma cell (lanes 3 and 6) lysates:1-3. Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:200).
4-6. Anti-NALCN/VGCNL1 (extracellular) Antibody, preincubated with NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022).
Expression of NALCN in rat hypothalamusImmunohistochemical staining of rat hypothalamus using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022). A. NALCN (green) staining appears in the supra-optic nucleus (SON). B. DAPI is used as the counterstain (blue). White arrows point at the sharp border between the SON and optic tract (OT). C. Merged images of A and B.
Expression of NALCN in rat PC12 cellsCell surface detection of NALCN in intact living rat pheochromocytoma PC12 cells. Extracellular staining of cells using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. Merged image of extracellular staining with live image of the cells.
- Lu, B. et al. (2007) Cell 129, 371.
- Gilon, P. and Rorsman, P. (2009) EMBO Rep. 10, 963.
- Swayne, L.A. et al. (2009) EMBO Rep. 10, 873.
- Swayne, L.A. et al. (2010) Islets 2, 54.
The existence of resting K+ conductance has been known for some time. Only recently has the Nalcn (sodium leak channel non-selective protein) been found responsible for the resting Na+ conductance1.
Nalcn is a member of the NaV and CaV channel family which have four homologous repeats of six transmembrane domains. One structural difference is that Nalcn is less positively charged in the voltage sensing segment1. The protein forms voltage-independent non-inactivating channel which is permeable to Na+, K+ and Ca2+ ions1,2.
Evidence suggests that Nalcn is a non-redundant and thus essential channel since knock-out mice display disrupted respiratory rhythm and die within 24 hours of birth. In addition, isolated neurons from these mice have decreased excitability1.
The channel is extensively expressed in the central nervous system1, heart, pituitary and adrenal glands and pancreatic islet cells2,3.
In pancreatic islets, Nalcn is activated by acetylcholine via its physical association with M3 muscarinic receptor, and potentiates insulin secretion induced by glucose uptake2-4.
Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022) is a highly specific antibody directed against an epitope of rat Sodium leak channel non-selective protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NALCN from mouse, rat, and human samples.
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